WEKO3
アイテム
{"_buckets": {"deposit": "9e20f8c0-4ab5-42c9-a1ad-c4503093ecf1"}, "_deposit": {"created_by": 1, "id": "27618", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "27618"}, "status": "published"}, "_oai": {"id": "oai:kumadai.repo.nii.ac.jp:00027618", "sets": ["414"]}, "author_link": ["157754", "157658", "157752", "157659", "157753"], "item_15_alternative_title_22": {"attribute_name": "その他の言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Establishment of an inducible M12-Tet-Off pTRE2pur FLAG mTRAF3 cell"}]}, "item_15_alternative_title_23": {"attribute_name": "タイトル(ヨミ)", "attribute_value_mlt": [{"subitem_alternative_title": "M12 Tet Off pTRE2pur FLAG mTRAF3 サイボウカブ ノ ジュリツ"}]}, "item_15_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-03-30", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "38", "bibliographicPageStart": "27", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "熊本大学医学部保健学科紀要"}]}]}, "item_15_creator_3": {"attribute_name": "別言語の著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "田邊, 香野", "creatorNameLang": "ja"}, {"creatorName": "タナベ, カノ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Tanabe, Kano", "creatorNameLang": "en"}], "familyNames": [{"familyName": "田邊", "familyNameLang": "ja"}, {"familyName": "タナベ", "familyNameLang": "ja-Kana"}, {"familyName": "Tanabe", "familyNameLang": "en"}], "givenNames": [{"givenName": "香野", "givenNameLang": "ja"}, {"givenName": "カノ", "givenNameLang": "ja-Kana"}, {"givenName": "Kano", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "157658", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "坂本, 瞳", "creatorNameLang": "ja"}, {"creatorName": "サカモト, ヒトミ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Sakamoto, Hitomi", "creatorNameLang": "en"}], "familyNames": [{"familyName": "坂本", "familyNameLang": "ja"}, {"familyName": "サカモト", "familyNameLang": "ja-Kana"}, {"familyName": "Sakamoto", "familyNameLang": "en"}], "givenNames": [{"givenName": "瞳", "givenNameLang": "ja"}, {"givenName": "ヒトミ", "givenNameLang": "ja-Kana"}, {"givenName": "Hitomi", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "157753", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "乾, 誠治", "creatorNameLang": "ja"}, {"creatorName": "イヌイ, セイジ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Inui, Seiji", "creatorNameLang": "en"}], "familyNames": [{"familyName": "乾", "familyNameLang": "ja"}, {"familyName": "イヌイ", "familyNameLang": "ja-Kana"}, {"familyName": "Inui", "familyNameLang": "en"}], "givenNames": [{"givenName": "誠治", "givenNameLang": "ja"}, {"givenName": "セイジ", "givenNameLang": "ja-Kana"}, {"givenName": "Seiji", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "157659", "nameIdentifierScheme": "WEKO"}]}]}, "item_15_description_17": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_15_description_46": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "論文(Article)", "subitem_description_type": "Other"}]}, "item_15_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "IL-4 induces IgE class switching in B cells together with CD40 stimulation. Studies on the signal transduction mechanism downstream of IL-4 receptor have revealed that NF-κB as well as Jak-STAT pathway plays an important role. We showed the possibility that alternative pathway of NF-κB was activated after IL-4 stimulation of murine B cell line M12. Alternative pathway of NF-κB is strictly regulated by constant degradation of NIK, an essential molecule for NF-κB activation. TRAF3 is the key molecule to keep the alternative pathway in an inactive state. TRAF3 is also regulated by the constant degradation by ubiquitin-proteasome system. To elucidate the mechanism of the TRAF3 degradation, we prepared Tet-Off gene expression system in M12 to inducibly express TRAF3 which was toxic when overexpressed in cells. Firstly, pTet-Off vector was introduced into M12 cells by electroporation and G418 resistant clones were selected which harbored pTet-Off. Clones were further selected to show high responsiveness to Doxycyclin withdrawal using transient expression of luciferase vector. One of the clones, C\u0027\u0027 , was transfected with pTRE2pur-mTRAF3 vector to obtain FLAG-tagged mTRAF3 expressing cells upon induction. One clone, C\u0027\u0027 -1\u0027 , was successfully established which expressed mTRAF3 in an inducible manner.", "subitem_description_type": "Other"}]}, "item_15_publisher_36": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "熊本大学"}]}, "item_15_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "18807151", "subitem_source_identifier_type": "ISSN"}]}, "item_15_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12010572", "subitem_source_identifier_type": "NCID"}]}, "item_15_subject_20": {"attribute_name": "日本十進分類法", "attribute_value_mlt": [{"subitem_subject": "463", "subitem_subject_scheme": "NDC"}]}, "item_15_text_18": {"attribute_name": "形態", "attribute_value_mlt": [{"subitem_text_value": "1588446 bytes"}]}, "item_15_text_47": {"attribute_name": "資源タイプ・ローカル", "attribute_value_mlt": [{"subitem_text_value": "紀要論文"}]}, "item_15_text_48": {"attribute_name": "資源タイプ・NII", "attribute_value_mlt": [{"subitem_text_value": "Departmental Bulletin Paper"}]}, "item_15_text_49": {"attribute_name": "資源タイプ・DCMI", "attribute_value_mlt": [{"subitem_text_value": "text"}]}, "item_15_text_50": {"attribute_name": "資源タイプ・ローカル表示コード", "attribute_value_mlt": [{"subitem_text_value": "02"}]}, "item_15_text_76": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_text_value": "http://hdl.handle.net/2298/27461"}]}, "item_15_version_type_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "田邊, 香野"}], "nameIdentifiers": [{"nameIdentifier": "157752", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "坂本, 瞳", "creatorNameLang": "ja"}, {"creatorName": "サカモト, ヒトミ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Sakamoto, Hitomi", "creatorNameLang": "en"}], "familyNames": [{"familyName": "坂本", "familyNameLang": "ja"}, {"familyName": "サカモト", "familyNameLang": "ja-Kana"}, {"familyName": "Sakamoto", "familyNameLang": "en"}], "givenNames": [{"givenName": "瞳", "givenNameLang": "ja"}, {"givenName": "ヒトミ", "givenNameLang": "ja-Kana"}, {"givenName": "Hitomi", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "157753", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "乾, 誠治"}], "nameIdentifiers": [{"nameIdentifier": "157754", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-03-02"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "MH0009_027-038.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "MH0009_027-038.pdf", "url": "https://kumadai.repo.nii.ac.jp/record/27618/files/MH0009_027-038.pdf"}, "version_id": "33a9144d-3fb9-4828-ac8a-3697e5b52827"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Tet-Off system", "subitem_subject_scheme": "Other"}, {"subitem_subject": "M12 cell", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TRAF3", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NF-kB", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立"}]}, "item_type_id": "15", "owner": "1", "path": ["414", "1260"], "permalink_uri": "http://hdl.handle.net/2298/27461", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-04-18"}, "publish_date": "2013-04-18", "publish_status": "0", "recid": "27618", "relation": {}, "relation_version_is_last": true, "title": ["M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立"], "weko_shared_id": -1}
M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立
http://hdl.handle.net/2298/27461
http://hdl.handle.net/2298/2746184c0f86e-da97-4402-b63b-3a0a448941bf
名前 / ファイル | ライセンス | アクション |
---|---|---|
MH0009_027-038.pdf (1.6 MB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2013-04-18 | |||||
タイトル | ||||||
タイトル | M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | Tet-Off system, M12 cell, TRAF3, NF-kB | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
田邊, 香野
× 田邊, 香野× 坂本, 瞳× 乾, 誠治 |
|||||
別言語の著者 |
田邊, 香野
× 田邊, 香野× 坂本, 瞳× 乾, 誠治 |
|||||
内容記述 | ||||||
内容記述 | IL-4 induces IgE class switching in B cells together with CD40 stimulation. Studies on the signal transduction mechanism downstream of IL-4 receptor have revealed that NF-κB as well as Jak-STAT pathway plays an important role. We showed the possibility that alternative pathway of NF-κB was activated after IL-4 stimulation of murine B cell line M12. Alternative pathway of NF-κB is strictly regulated by constant degradation of NIK, an essential molecule for NF-κB activation. TRAF3 is the key molecule to keep the alternative pathway in an inactive state. TRAF3 is also regulated by the constant degradation by ubiquitin-proteasome system. To elucidate the mechanism of the TRAF3 degradation, we prepared Tet-Off gene expression system in M12 to inducibly express TRAF3 which was toxic when overexpressed in cells. Firstly, pTet-Off vector was introduced into M12 cells by electroporation and G418 resistant clones were selected which harbored pTet-Off. Clones were further selected to show high responsiveness to Doxycyclin withdrawal using transient expression of luciferase vector. One of the clones, C'' , was transfected with pTRE2pur-mTRAF3 vector to obtain FLAG-tagged mTRAF3 expressing cells upon induction. One clone, C'' -1' , was successfully established which expressed mTRAF3 in an inducible manner. | |||||
書誌情報 |
熊本大学医学部保健学科紀要 巻 9, p. 27-38, 発行年 2013-03-30 |
|||||
ISSN | ||||||
収録物識別子 | 18807151 | |||||
NCID | ||||||
収録物識別子 | AA12010572 | |||||
フォーマット | ||||||
内容記述 | application/pdf | |||||
形態 | ||||||
1588446 bytes | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
日本十進分類法 | ||||||
主題 | 463 | |||||
その他の言語のタイトル | ||||||
その他のタイトル | Establishment of an inducible M12-Tet-Off pTRE2pur FLAG mTRAF3 cell | |||||
タイトル(ヨミ) | ||||||
その他のタイトル | M12 Tet Off pTRE2pur FLAG mTRAF3 サイボウカブ ノ ジュリツ | |||||
出版者 | ||||||
出版者 | 熊本大学 | |||||
資源タイプ | ||||||
内容記述 | 論文(Article) | |||||
資源タイプ・ローカル | ||||||
紀要論文 | ||||||
資源タイプ・NII | ||||||
Departmental Bulletin Paper | ||||||
資源タイプ・DCMI | ||||||
text | ||||||
資源タイプ・ローカル表示コード | ||||||
02 |