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フローサイトメトリーによるフィルター処理血液製剤中の残存白血球数の測定
http://hdl.handle.net/2298/16774
http://hdl.handle.net/2298/167747ef93316-90b4-4c27-844c-7449ead832a9
名前 / ファイル | ライセンス | アクション |
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MT0005_027-033.pdf (1.9 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-11-09 | |||||
タイトル | ||||||
タイトル | フローサイトメトリーによるフィルター処理血液製剤中の残存白血球数の測定 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | Blood product, Residual leukocyte, Flowcytometry | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
石井, 俊徳
× 石井, 俊徳× 大橋, 綾子× 篠原, 都子× 伊東, 孝子× 楠本, 行彦 |
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別言語の著者 |
石井, 俊徳
× 石井, 俊徳× Ohashi, Ayako× Shinohara, Miyako× Itoh, Takako× Kusumoto, Yukihiko |
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内容記述 | ||||||
内容記述 | 私達はフローサイトメトリー(FCM)を用い、フィルター法により作製した白血球除去血液製剤中の微量白血球の測定法の確立を試みた。 | |||||
内容記述 | ||||||
内容記述 | We detected a very small number of leukocytes in blood products which couldn't be counted with electronic haemocyte counter by the method of flowcytometry and nuclear staining with propidium iodide. This method revealed that the number of residual leukocyte was on the level of 10 in a concentrated red cell product, in the level\ of 10 in an washed red cell products and on the level of 10 in a filtered red cell product. In case of platelet products, the number of residual leukocyte was on the level of 10 in a concentrated platelet product and on the level of 10 in a filtered platelet product. Residual leukocytes in filtered blood products fell below the level of 5x10 which was critical number of alloimmunity induction. Blood preservation for 7 days didn't improved filtration rate of leukocytes. Filtration made leukocyte fractions relatively Iymphopenic and granulocytotic in comparison with ones in original products. We expect that improvement of filtration capacity to remove granulocytes will make the number of residual leukocytes fall on the level of 10, which our method can fully deal with. |
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書誌情報 |
熊本大学医療技術短期大学部紀要 巻 5, p. 27-33, 発行年 1995-03-01 |
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ISSN | ||||||
収録物識別子 | 09181512 | |||||
NCID | ||||||
収録物識別子 | AN10401515 | |||||
フォーマット | ||||||
内容記述 | application/pdf | |||||
形態 | ||||||
1856287 bytes | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
日本十進分類法 | ||||||
主題 | 492.26 | |||||
その他の言語のタイトル | ||||||
その他のタイトル | Detection of Residual Leukocytes in Filtered Blood Products by Flowcytometry | |||||
タイトル(ヨミ) | ||||||
その他のタイトル | フローサイトメトリー ニ ヨル フィルター ショリ ケツエキ セイザイ チュウ ノ ザンゾン ハッケッキュウ スウ ノ ソクテイ | |||||
出版者 | ||||||
出版者 | 熊本大学 | |||||
資源タイプ | ||||||
内容記述 | 論文(Article) | |||||
資源タイプ・ローカル | ||||||
紀要論文 | ||||||
資源タイプ・NII | ||||||
Departmental Bulletin Paper | ||||||
資源タイプ・DCMI | ||||||
text | ||||||
資源タイプ・ローカル表示コード | ||||||
02 |