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Cessation of gastrulation is mediated by suppression of epithelial-mesenchymal transition at the ventral ectodermal ridge 太田, 将 山田, 源 ヤマダ, ゲン Yamada, Gen 山田 ヤマダ Yamada 源 ゲン Gen Ohta, Sho 鈴木, 堅太郎 スズキ, ケンタロウ Suzuki, Kentaro 鈴木 スズキ Suzuki 堅太郎 ケンタロウ Kentaro Tachibana, Katsuro Tachibana Katsuro 田中, 英明 タナカ, ヒデアキ Tanaka, Hideaki 田中 タナカ Tanaka 英明 ヒデアキ Hideaki 山田, 源 ヤマダ, ゲン Yamada, Gen 山田 ヤマダ Yamada 源 ゲン Gen 493.937 Chick Mouse Bmp Noggin Gastrulation Epithelial-mesenchymal transition (EMT) Ventral ectodermal ridge (VER) Tail Sonoporation application/pdf 論文(Article) In the gastrula stage embryo, the epiblast migrates toward the primitive streak and ingresses through the primitive groove. Subsequently, the ingressing epiblast cells undergo epithelial-mesenchymal transition (EMT) and differentiate into the definitive endoderm and mesoderm during gastrulation. However, the developmental mechanisms at the end of gastrulation have not yet been elucidated. Histological and genetic analyses of the ventral ectodermal ridge (VER), a derivative of the primitive streak, were performed using chick and mouse embryos. The analyses showed a continued cell movement resembling gastrulation associated with EMT during the early tailbud stage of both embryos. Such gastrulation-like cell movement was gradually attenuated by the absence of EMT during tail development. The kinetics of the expression pattern of noggin (Nog) and basal membrane degradation adjacent to the chick and the mouse VER indicated a correlation between the temporal and/or spatial expression of Nog and the presence of EMT in the VER. Furthermore, Nog overexpression suppressed EMT and arrested ingressive cell movement in the chick VER. Mice mutant in noggin displayed dysregulation of EMT with continued ingressive cell movement. These indicate that the inhibition of Bmp signaling by temporal and/or spatial Nog expression suppresses EMT and leads to the cessation of the ingressive cell movement from the VER at the end of gastrulation. http://dev.biologists.org/cgi/content/full/134/24/4315 Company of Biologists 2007-12 eng journal article VoR http://hdl.handle.net/2298/10388 https://kumadai.repo.nii.ac.jp/records/21964 10.1242/dev.008151 09501991 AA10667805 Development 134 24 4315 4324 https://kumadai.repo.nii.ac.jp/record/21964/files/Ohta_Development_2007.pdf application/pdf 6.7 MB 2020-03-02