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        <identifier>oai:kumadai.repo.nii.ac.jp:00024442</identifier>
        <datestamp>2023-09-13T09:20:14Z</datestamp>
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          <dc:title>生理活性を利用した海洋天然物の未同定生合成遺伝子の取得と物質生産</dc:title>
          <dcterms:alternative>Cloning and Expression of Marine Natural Product Biosynthetic Genes by Function Based Screening</dcterms:alternative>
          <dcterms:alternative>セイリ カッセイ オ リヨウ シタ カイヨウ テンネンブツ ノ ミ ドウテイ セイゴウセイ イデンシ ノ シュトク ト ブッシツ セイサン</dcterms:alternative>
          <jpcoar:creator>
            <jpcoar:creatorName xml:lang="ja">藤田, 雅紀</jpcoar:creatorName>
            <jpcoar:creatorName xml:lang="ja-Kana">フジタ, マサキ</jpcoar:creatorName>
            <jpcoar:creatorName xml:lang="en">Fujita, Masaki</jpcoar:creatorName>
            <jpcoar:familyName xml:lang="ja">藤田</jpcoar:familyName>
            <jpcoar:familyName xml:lang="ja-Kana">フジタ</jpcoar:familyName>
            <jpcoar:familyName xml:lang="en">Fujita</jpcoar:familyName>
            <jpcoar:givenName xml:lang="ja">雅紀</jpcoar:givenName>
            <jpcoar:givenName xml:lang="ja-Kana">マサキ</jpcoar:givenName>
            <jpcoar:givenName xml:lang="en">Masaki</jpcoar:givenName>
            <jpcoar:affiliation/>
          </jpcoar:creator>
          <jpcoar:creator>
            <jpcoar:creatorName>藤田, 雅紀</jpcoar:creatorName>
          </jpcoar:creator>
          <jpcoar:subject subjectScheme="NDC">468.8</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">海洋天然物</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">生合成</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">メタゲノム</jpcoar:subject>
          <datacite:description>application/pdf</datacite:description>
          <datacite:description descriptionType="Other">研究報告書</datacite:description>
          <datacite:description descriptionType="Other">海洋メタゲノムを利用した有用物質生産を目標に、メタゲノムライブラリの作成技術構築から生産物質の同定まで一貫して行い、本方法論が実現可能であることを示した。本研究を通して、高品質海洋メタゲノムライブラリを約10万クローン作製し、また300以上の色素生産クローンを取得した。またそのうち20以上のクローンについて、その生合成遺伝子と生産物の同定を行った。</datacite:description>
          <datacite:description descriptionType="Other">Metagenomics is a promising method to obtain useful genes from
environmental samples. In order to prove that metagenomics is applicable to produce marine natural products, high quality marine metagenomic library consist of about 100 thousands clones was constructed. More than 300 pigment producing clones were screened from the library and their biosynthetic genes and products were characterized.</datacite:description>
          <dc:publisher>熊本大学</dc:publisher>
          <datacite:date dateType="Issued">2010-04-01</datacite:date>
          <dc:language>jpn</dc:language>
          <dc:type rdf:resource="http://purl.org/coar/resource_type/c_18ws">research report</dc:type>
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          <jpcoar:identifier identifierType="HDL">http://hdl.handle.net/2298/17712</jpcoar:identifier>
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            <datacite:date dateType="Available">2020-03-02</datacite:date>
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