| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-09-19 |
| タイトル |
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タイトル |
Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
Melanoma, Nucleosome assembly protein 1-like4, Migration, Invasion, Slug, p21 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Satoru, Mizuhashi
Satoshi, Fukushima
Takayuki, Ishibashi
Haruka, Kuriyama
Toshihiro, Kimura
Hisashi, Kanemaru
Ikko, Kajihara
Katsunari, Makino
Azusa, Miyashita
Jun, Aoi
Kanako, Kita
Hironobu, Ihn
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| 内容記述 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Background: Melanoma is one of the deadliest skin cancers. The treatment of advanced melanoma has been dramatically improved by immune checkpoint inhibitors and targeted therapies. However, many patients still do not respond to these therapies.
Objective: To investigate whether NAP1L4 can be a new therapeutic target for melanoma.
Methods: Immunohistochemical analysis of human nevus and melanoma tissues was performed. Real-time RT-PCR and immunoblotting were performed using human samples and melanoma cell lines. Next, we examined the effect of NAP1L4 knockdown in melanoma cell lines using cell migration and invasion assays. To investigate the molecular mechanism related to these results, immunoblotting of p21 and Slug was examined. MMP-2 and MMP-9 activity assays were also performed. Further, pathway analysis between NAP1L4 and MMP-2 was performed. Finally, the effects of NAP1L4 knockdown on cell proliferation, apoptosis, and cell cycle were analyzed.
Results: NAP1L4 was overexpressed in melanoma tissues compared to the nevus tissue. NAP1L4 knockdown reduced melanoma cell migration and invasion. NAP1L4 knockdown upregulated p21 and downregulated Slug expression in melanoma cells. NAP1L4 knockdown decreased the active levels of MMP-2 in the supernatant from melanoma cells. NAP1L4 knockdown inhibited apoptosis in camptothecin-induced DNA damage, induced cell cycle arrest at the G1/S phase, and inhibited cell proliferation.
Conclusions: NAP1L4 may play a role in cell migration and invasion in melanoma cells through the regulation of Slug. We propose that NAP1L4 can be a new therapeutic target for proliferation and invasion of melanoma cells. |
| 書誌情報 |
en : Journal of Dermatological Science
巻 102,
号 1,
p. 16-24,
発行年 2021-04-01
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| ISSN |
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収録物識別子 |
0923-1811 |
| DOI |
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関連タイプ |
isVersionOf |
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関連識別子 |
https://doi.org/10.1016/j.jdermsci.2021.02.001 |
| 権利 |
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権利情報 |
(C) 2021 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 出版者 |
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出版者 |
Elsevier |
j_jdermsci_202102001.pdf (1.1 MB)
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