| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-11-15 |
| タイトル |
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タイトル |
Fluorescent image-based high-content screening of extracts of natural resources for cell cycle inhibitors and identification of a new sesquiterpene quinone from the sponge, Dactylospongia metachromia |
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言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
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主題 |
Cell cycle, Fucci, Natural product, Marine sponge, Sesquiterpene quinone |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
Yuki, Hitora
Ai, Sejiyama
Koyo, Honda
Yuji, Ise
Fitje, Losung
Remy, E. P. Mangindaan
塚本, 佐知子
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| 内容記述 |
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内容記述 |
Natural products are important sources for drug development. Discovery of natural products that inhibit cell cycle progression significantly contributes to the progress of cancer biology and the development of new antitumor agents. In this study, cell cycle inhibitory activity was evaluated with our extract library of natural resources, including marine invertebrates, fungi, and bacteria, using HeLa/Fucci2 cells which allow classification of the cell cycle phases of living cells. Screening of the extract library revealed that the extract of the marine sponge Dactylospongia metachromia inhibited cell cycle progression at S/G2/M phases. Bioassay-guided fractionation afforded a new sesquiterpene quinone, neoisosmenospongine (1), and four known compounds, nakijiquinone I, N, and Q (2-4) and (-)-dictyoceratin-C (5). The chemical structure of 1 was elucidated by interpretating the NMR and mass spectroscopic data, and the absolute configuration was determined by comparison of the experimental and calculated ECD spectra. Fluorescent imaging of HeLa/Fucci2 cells revealed that 1-4 inhibited the cell cycle progression at S/G2/M phases. This study demonstrated that fluorescent image-based high-content screening using HeLa/Fucci2 cells is an effective approach for isolating cell cycle inhibitors from natural resources. |
| 書誌情報 |
en : Bioorganic & Medicinal Chemistry
巻 31,
p. 115968,
発行年 2021-02-01
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| ISSN |
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収録物識別子 |
0968-0896 |
| DOI |
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関連タイプ |
isVersionOf |
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関連識別子 |
https://doi.org/10.1016/j.bmc.2020.115968 |
| 権利 |
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権利情報 |
(C) 2020 Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 著者版フラグ |
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出版タイプ |
AM |
| 出版者 |
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出版者 |
Elsevier |
|
言語 |
en |
j_bmc_2020_115968.pdf (1.1 MB)
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