| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-09-27 |
| タイトル |
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タイトル |
Cell-penetrating Albumin Enhances the Sublingual Delivery of Antigens through Macropinocytosis |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
Sublingual immunotherapy, Human serum albumin, Cell-penetrating peptide |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Maeda, Hitoshi
Ichimizu, Shota
Watanabe, Hiroshi
Hamasaki, Keisuke
Chikamatsu, Mayuko
Murata, Ryota
Yumoto, Nao
Seki, Takahiro
Katsuki, Hiroshi
Otagiri, Masaki
Maruyama, Toru
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| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
Innovations in oral immunotherapy have greatly advanced the therapeutic control of allergies. However, these therapeutic effects suffer from the fact that the amount of antigen delivered to antigen-presenting cells is limited given the formulations that are currently available. We recently designed a cell-penetrating albumin and found that this modified albumin enters cells via the induction of macropinocytosis. Herein, we report on a novel system for delivering antigens based on cell-penetrating albumin-inducible macropinocytosis that allows larger amounts of antigens to be delivered to antigen-presenting cells. A treatment with cell-penetrating albumin significantly increased the permeability of ovalbumin (45 kDa) or dextran (2000 kDa) on monolayers derived from human oral squamous carcinoma cells. Flow cytometric analyses showed that the cell-penetrating albumin treatment resulted in a significant elevation in the amount of dextran that was delivered to two types of antigen-presenting cells. Finally, mice that had been sensitized by Japanese cedar pollen extract (JCPE) and cell-penetrating albumin showed a decline in the frequency of nose-rubbing against a subsequent intranasal administration of JCPE. These findings suggest that the sublingual administration of cell-penetrating albumin efficiently delivers antigens to antigen-presenting cells via the induction of macropinocytosis, resulting in an enhancement in the therapeutic effect of sublingual immunotherapy. |
| bibliographic_information |
en : International Journal of Biological Macromolecules
巻 221,
p. 1439-1452,
発行年 2022-11-30
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| item_16_source_id_7 |
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収録物識別子 |
0141-8130 |
| 権利 |
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権利情報 |
(C) 2022 Elsevier B.V. All rights reserved. |
| 権利 |
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権利情報 |
This manuscript version is made available under the CC-BY-NC-ND 4.0 license. https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 出版者 |
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出版者 |
Elsevier |
| 関連 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.ijbiomac.2022.09.132 |
10.1016_j.ijbiomac.2022.09.132.pdf (3.4 MB)
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