| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-10-17 |
| タイトル |
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タイトル |
Molecular and pharmacological characterization of zebrafish ‘contractile’ and ‘inhibitory’ prostanoid receptors |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
Prostaglandins, Thromboxanes, GPCR, Pharmacology, Signal transduction |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Ryo, Iwasaki
Kyoshiro, Tsuge
Kazushi, Morimoto
Tomoaki, Inazumi
Osamu, Kawahara
Atsuo, Kawahara
Soken, Tsuchiya
Yukihiko, Sugimoto
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| 内容記述 |
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内容記述 |
Prostanoids comprising prostaglandins (PGs) and thromboxanes (TXs) have been shown to play physiological and pathological roles in zebrafish. However, the molecular basis of zebrafish prostanoid receptors has not been established. Here, we demonstrate that there exist at least five ‘contractile’ (Ca2+-mobilizing) and one ‘inhibitory’ (Gi-coupled) prostanoid receptors in zebrafish; five ‘contractile’ receptors consisting of two PGE2 receptors (EP1a and EP1b), two PGF2α receptors (FP1 and FP2), and one TXA2 receptor TP, and one ‘inhibitory’ receptor, the PGE2 receptor EP3. [3H]PGE2 specifically bound to the membranes of cells expressing zebrafish EP1a, EP1b and EP3 with a Kd of 4.8, 1.8 and 13.6 nM, respectively, and [3H]PGF2α specifically bound to the membranes of cells expressing zebrafish FP1 and FP2, with a Kd of 6.5 and 1.6 nM, respectively. U-46619, a stable agonist for human and mouse TP receptors, significantly increased the specific binding of [35S]GTPγS to membranes expressing the zebrafish TP receptor. Upon agonist stimulation, all six receptors showed an increase in intracellular Ca2+ levels, although the increase was very weak in EP1b, and pertussis toxin abolished only the EP3-mediated response. Zebrafish EP3 receptor also suppressed forskolin-induced cAMP formation in a pertussis toxin-sensitive manner. In association with the low structural conservation with mammalian receptors, most agonists and antagonists specific for mammalian EP1, EP3 and TP failed to work on each corresponding zebrafish receptor. This work provides further insights into the diverse prostanoid actions mediated by their receptors in zebrafish. |
| bibliographic_information |
en : Biochemical and Biophysical Research Communications
巻 438,
号 2,
p. 353-358,
発行年 2013-08-23
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| item_16_source_id_7 |
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収録物識別子 |
0006-291X |
| item_16_relation_11 |
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関連タイプ |
isVersionOf |
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関連識別子 |
https://doi.org/10.1016/j.bbrc.2013.07.075 |
| 権利 |
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権利情報 |
(C) 2013 Elsevier Inc. All rights reserved. |
| 権利 |
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権利情報 |
This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 出版タイプ |
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出版タイプ |
AM |
| 出版者 |
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出版者 |
Elsevier |
|
言語 |
en |
10.1016_j.bbrc.2013.07.075.pdf (1.8 MB)
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