| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-12-09 |
| タイトル |
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タイトル |
Single Nanosecond Pulsed Electric Field Effects on Embryonic Development of the Medaka Fish |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
Embryonic development, ns pulsed electric field, blastomere cells, in-vivo study |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Dong Koo, Kang
S. Hamid R., Hosseini
Eri, Shiraishi
Masato, Yamanaka
Hidenori, Akiyama
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| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
The effects of nanosecond pulsed electric field (ns PEF) on the egg development of the medaka fish are investigated. A pulsed power modulator using a magnetic pulse compression circuit was employed to generate pulses of 0.5-20 kV pulses. Fertilized eggs of strain d-rR medaka were used. The ages of the experimental eggs were new laid, 24-h postfertilization, and 48-h postfertilization. Propidium iodide and fluorescein isothiocyanate (FITC)-dextran conjugate, and a fluorescence microscope were used to study the effects of ns PEF on the blastomeres and the extent of egg structure damage. The FITC was injected to experimental eggs by a microinjection system, which can deliver nanoliters of FITC without producing any damage to the egg structure. Each egg was set at the middle of a 2- or 4-mm cuvette, and a single pulse was applied. The control eggs were sham treated. After the pulse application, the eggs were observed under the fluorescence microscope until they hatched or died. By applying low electric field pulses (10 kV/cm), the effects were minimal and temporary. These eggs could recover and grow as fishes. By increasing the electric field (20 kV/cm), embryos showed abnormal growth with deformed body structure or missing organs. For higher electric fields (over 30 kV/cm), immediate extended damage were observed. The research was motivated by its application in regenerative medicine to control embryonic-stem-cell differentiation and proliferation. |
| bibliographic_information |
en : IEEE Transactions on Plasma Science
巻 40,
号 10,
p. 2379-2387,
発行年 2012-10
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| item_16_source_id_7 |
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収録物識別子 |
0093-3813 |
| item_16_relation_11 |
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関連タイプ |
isVersionOf |
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関連識別子 |
https://doi.org/10.1109/TPS.2012.2192506 |
| 権利 |
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権利情報 |
(C) 2012 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 出版者 |
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出版者 |
IEEE |
10.1109_TPS.2012.2192506.pdf (1.7 MB)
