| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-22 |
| タイトル |
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タイトル |
Novel cyclic peptides facilitating transcellular blood-brain barrier transport of macromolecules in vitro and in vivo |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
Cyclic peptide, Phage display technology, Brain delivery, Macromolecule |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Shunsuke, Yamaguchi
Shingo, Ito
Takeshi, Masuda
Pierre-Olivier, Couraud
Sumio, Ohtsuki
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| 内容記述 |
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内容記述 |
Brain delivery of nanoparticles and macromolecular drugs depends on blood-brain barrier (BBB)-permeable carriers. In this study, we searched for cyclic heptapeptides facilitating BBB permeation of M13 phages by phage library screening using a transcellular permeability assay with hCMEC/D3 cell monolayers, a human BBB model. The M13 phage, which is larger than macromolecular drugs and nanoparticles, served as a model macromolecule. The screen identified cyclic heptapeptide SLSHSPQ (SLS) as a human BBB-permeable peptide. The SLS-displaying phage (SLS-phage) exhibited improved permeation across the cell monolayer of monkey and rat BBB co-culture models. The SLS-phage internalized into hCMEC/D3 cells via macropinocytosis and externalized via the exosome excretion pathway. SLS-phage distribution into brain parenchyma was observed in mice after intravenous administration. Moreover, liposome permeated across the BBB as cyclic SLS peptide conjugates. In conclusion, the cyclic SLS heptapeptide is a novel carrier candidate for brain delivery of macromolecular drugs and nanoparticles. |
| bibliographic_information |
en : Journal of Controlled Release
巻 321,
p. 744-755,
発行年 2020-05-10
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| item_16_source_id_7 |
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収録物識別子 |
0168-3659 |
| item_16_relation_11 |
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関連タイプ |
isVersionOf |
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関連識別子 |
https://doi.org/10.1016/j.jconrel.2020.03.001 |
| 権利 |
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権利情報 |
(C) 2020 Elsevier B.V. All rights reserved. |
| 権利 |
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権利情報 |
This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 出版タイプ |
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出版タイプ |
AM |
| 出版者 |
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出版者 |
Elsevier |
|
言語 |
en |
10.1016_j.jconrel.2020.03.001.pdf (1.5 MB)
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