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BMPアンタゴニストDanによるニワトリ内耳パターン形成および内リンパ管・嚢の形態形成の制御
http://hdl.handle.net/2298/8123
http://hdl.handle.net/2298/8123f09d1a36-21a8-4285-bb0b-98cdd812c339
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
22-1564.pdf (4.3 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-08-13 | |||||
| タイトル | ||||||
| タイトル | BMPアンタゴニストDanによるニワトリ内耳パターン形成および内リンパ管・嚢の形態形成の制御 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題 | 内耳, 発生, ニワトリ胚, 内耳発生 | |||||
| 資源タイプ | ||||||
| 資源タイプ | thesis | |||||
| 著者 |
山西, 貴大
× 山西, 貴大 |
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| 別言語の著者 |
山西, 貴大
× 山西, 貴大 |
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| 内容記述 | ||||||
| 内容記述 | Bone morphogenetic proteins (BMPs) and BMP antagonists localized to specific regions have been known to interact each other and play critical roles in the development of multiple organs. Bmp4 is expressed in the prospective sensory epithelium of the chicken inner ear. It is suggested that there is the spatial-temporal regulation between BMP4 and its antagonists for the pattern formation and morphogenesis. We demonstrated that Dan acts as an antagonist by binding to BMP4 with high affinity. Dan expression was restricted to the dorso-medial region of the otic epithelium and maintained in the endolymphatic duct and sac (ed/es) developed from the region. To analyze roles of Dan in the chicken inner ear development, we performed in ovo electroporation of Dan-siRNA and constitutively active form of BMP receptor type IB (BMPRIB) for loss-of-function. Remarkable reductions of the ed/es in electroporated inner ears were demonstrated. Preceding them, we confirmed that the expression of Msx1, an early marker for the ed/es, was weakened and extended ventrally afterwards. In addition, the Nkx5.1 expression that is restricted to the dorso-lateral region was weakened and detected in the medial region ectopically. Furthermore, by knock down of Dan, apoptotic cells were observed in the ventro-medial region of otic vesicles where BMP4 expresses strongly at the stage. These results suggest that Dan regulates the pattern formation along dorsal-ventral and medial-lateral axes and controls apoptosis induced by BMP signals, and later, regulates the morphogenesis of the ed/es in the chicken inner ear development. | |||||
| 書誌情報 | 発行年 2006-03-24 | |||||
| フォーマット | ||||||
| 内容記述 | application/pdf, application/pdf, text/plain | |||||
| 形態 | ||||||
| 4289507 bytes, 60948 bytes, 39047 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 日本十進分類法 | ||||||
| 主題 | 377.5 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | BMP antagonist Dan acts as a critical regulator of the patterning and morphogenesis of the ed/es in the chick inner ear development | |||||
| タイトル（ヨミ） | ||||||
| その他のタイトル | BMP アンタゴニスト Dan ニ ヨル ニワトリ ナイジ パターン ケイセイ オヨビ ナイリンパカン ノウ ノ ケイタイ ケイセイ ノ セイギョ | |||||
| 出版者 | ||||||
| 出版者 | 熊本大学 | |||||
| 資源タイプ | ||||||
| 内容記述 | 学位論文(Thesis) | |||||
| 資源タイプ・ローカル | ||||||
| 博士論文 | ||||||
| 資源タイプ・NII | ||||||
| Thesis or Dissertation | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 学位番号 | ||||||
| 甲博医第1564号 | ||||||
