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Heat shock protein 105 を標的とした siRNA による癌細胞アポトーシスの誘導
http://hdl.handle.net/2298/8124
http://hdl.handle.net/2298/8124fb330d87-4525-41d8-bb12-f6c76d939799
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
22-1565.pdf (3.5 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-08-13 | |||||
| タイトル | ||||||
| タイトル | Heat shock protein 105 を標的とした siRNA による癌細胞アポトーシスの誘導 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題 | 癌特異抗原, 腫瘍, 細胞株, アポトーシス | |||||
| 資源タイプ | ||||||
| 資源タイプ | thesis | |||||
| 著者 |
保坂, 征司
× 保坂, 征司 |
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| 別言語の著者 |
保坂, 征司
× 保坂, 征司 |
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| 内容記述 | ||||||
| 内容記述 | We previously reported that the heat shock protein 105 (HSP105), identified by a serological analysis of the recombinant cDNA expression library (SEREX) using the serum from a pancreatic cancer patient, was overexpressed in various human tumors and the testis of adult men by immunohistochemical analysis. In the present study, to elucidate the biological function of HSP105 protein in cancer cells, we first established NIH3T3 cells overexpressing murine HSP105 (NIH3T3-HSP105). The NIH3T3-HSP105 cells acquired resistance to apoptosis induced by heat shock or doxorubicin. The small interfering RNA (siRNA)-mediated suppression of HSP105 protein expression induced apoptosis in human cancer cells but not in fibroblasts. By a combination of siRNA introduction and doxorubicin or heat shock treatment, apoptosis was induced synergistically in a human colon cancer cell line, HCT116 cells. In vivo, siRNA inoculation into the human gastric cancer cell line KATO-III established in the flank of an NOD SCID mouse suppressed the tumor growth. This siRNA-induced apoptosis was mediated through caspases, but not the p53 tumor suppressor protein, even though the HSP105 protein was bound to wild type p53 protein in HCT116 cells. These findings suggest that the constitutive overexpression of HSP105 in cancer cells is involved in malignant transformation by protecting tumor cells from apoptosis. HSP105 may thus be a novel target molecule for cancer therapy and a treatment regimen using synthetic siRNA to suppress the expression of HSP105 protein may provide a new strategy for cancer therapy. | |||||
| 書誌情報 | 発行年 2006-03-24 | |||||
| フォーマット | ||||||
| 内容記述 | application/pdf, application/pdf, text/plain | |||||
| 形態 | ||||||
| 3486164 bytes, 60947 bytes, 45451 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 日本十進分類法 | ||||||
| 主題 | 377.5 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | Synthetic small interfering RNA targeting heat shock protein 105 induces apoptosis of various cancer cells both in vitro and in vivo | |||||
| タイトル（ヨミ） | ||||||
| その他のタイトル | Heat shock protein 105 オ ヒョウテキ トシタ siRNA ニ ヨル ガンサイボウ アポトーシス ノ ユウドウ | |||||
| 出版者 | ||||||
| 出版者 | 熊本大学 | |||||
| 資源タイプ | ||||||
| 内容記述 | 学位論文(Thesis) | |||||
| 資源タイプ・ローカル | ||||||
| 博士論文 | ||||||
| 資源タイプ・NII | ||||||
| Thesis or Dissertation | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 学位番号 | ||||||
| 甲博医第1565号 | ||||||
