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Sodium Orthovanadate Enhances Proliferation of Progenitor cells in the Adult Rat Subventricular Zone after Focal Cerebral Ischemia
http://hdl.handle.net/2298/8206
http://hdl.handle.net/2298/82060e9b1553-f652-4b78-a9c8-98ada395a617
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
22-1573.pdf (5.9 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2014-08-13 | |||||
| タイトル | ||||||
| タイトル | Sodium Orthovanadate Enhances Proliferation of Progenitor cells in the Adult Rat Subventricular Zone after Focal Cerebral Ischemia | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | thesis | |||||
| 著者 |
松元, 淳
× 松元, 淳 |
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| 別言語の著者 |
松元, 淳
× 松元, 淳 |
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| 内容記述 | ||||||
| 内容記述 | Neuronal progenitor cells able to produce new neuron and glia persist in the adult central nervous system (CNS). Their proliferation is up-regulated by growth factors or cytokines under some pathological conditions including ischemia. As sodium orthovanadate (SOV), a protein tyrosine phosphatase inhibitor, can up-regulate tyrosine kinase-linked growth factors receptor signaling via the inhibition of tyrosine residue dephosphorylation, it may be capable of enhancing progenitor cell. To investigate the effect of SOV on progenitor cells in the subventricular zone (SVZ), we injected rats intraperitoneally with 50 mg/kg Bromodeoxyuridine (BrdU) and SOV (12.5 or 25 mM) or BrdU and Saline (control) on days 1-7 after middle cerebral artery occlusion. The density of BrdU-positive cells in the ipsilateral SVZ showed a significant SOV dose-dependent increase. This effect was found only in the ipsilateral- but not the contralateral SVZ, and not in non-ischemic rats. Double-immunolabeling with BrdU and double cortin (Dcx), a marker of migrating neuroblast, revealed that the density of double positive cells increased significantly in a SOV dose-dependent manner. TUNEL staining suggested that the SOV-induced increase was not due to anti-apoptotic effects. Treatment with SOV also significantly increased the density of cells positive for BrdU and phosphorylated Akt and BrdU and phosphorylated ERK. We postulate that ischemia triggers off the proliferation of SVZ cells by bioactive factors such as growth factors and that SOV enhances the proliferation of only triggered-off SVZ cells with Akt and ERK activation. Our findings suggest that SOV may aid in the self-repair of the post-ischemic CNS. |
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| 書誌情報 | 発行年 2007-03-27 | |||||
| フォーマット | ||||||
| 内容記述 | application/pdf, application/pdf, text/plain | |||||
| 形態 | ||||||
| 5881909 bytes, 60939 bytes, 84394 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 日本十進分類法 | ||||||
| 主題 | 377.5 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | ラット一過性中大脳動脈閉塞モデルを用いた、オルソバナジン酸ナトリウム投与による subventricular zone の神経前駆細胞の増加 | |||||
| タイトル（ヨミ） | ||||||
| その他のタイトル | ラット イッカセイ チュウ ダイノウ ドウミャク ヘイソク モデル オ モチイタ オルソバナジンサン ナトリウム トウヨ ニ ヨル subventricular zone ノ シンケイ ゼンク サイボウ ノ ゾウカ | |||||
| 出版者 | ||||||
| 出版者 | 熊本大学 | |||||
| 資源タイプ | ||||||
| 内容記述 | 学位論文(Thesis) | |||||
| 資源タイプ・ローカル | ||||||
| 博士論文 | ||||||
| 資源タイプ・NII | ||||||
| Thesis or Dissertation | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| 学位番号 | ||||||
| 甲博医第1573号 | ||||||
