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新規ヒト癌関連抗原 Secreted protein acidic and rich in cysteine (SPARC) の癌の診断と治療における有用性
http://hdl.handle.net/2298/8248
http://hdl.handle.net/2298/82489f3a65fb-60ea-4f55-baa1-157bb6cf3a00
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
22-1579.pdf (4.7 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2014-08-12 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 新規ヒト癌関連抗原 Secreted protein acidic and rich in cysteine (SPARC) の癌の診断と治療における有用性 | |||||||||||
| 言語 | ja | |||||||||||
| タイトル | ||||||||||||
| タイトル | シンキ ヒト ガン カンレン コウゲン Secreted protein acidic and rich in cysteine (SPARC) ノ ガン ノ シンダン ト チリョウ ニ オケル ユウヨウセイ | |||||||||||
| 言語 | ja-Kana | |||||||||||
| タイトル | ||||||||||||
| タイトル | Secreted protein acidic and rich in cysteine, an ideal cancer-associated antigen useful for serum diagnosis and immunotherapy of cancers, identified using cDNA microarray analysis | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 主題 | 抗腫瘍免疫, 抗原, 遺伝子 メラノーマ |
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| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||||||
| 資源タイプ | thesis | |||||||||||
| 著者 |
生田, 義明
× 生田, 義明
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| 内容記述 | ||||||||||||
| 内容記述 | Purpose: To establish effective diagnostic method and anti-tumor immunotherapy for several cancers, we tried to identify a novel tumor-associated antigen. Experimental design: We analyzed data obtained from cDNA microarray analysis of diffuse gastric cancer tissue and 29 normal tissues to find a novel tumor-associated antigen, secreted protein acidic and rich in cysteine (SPARC). Because melanoma cells were reported to express a large amount of SPARC, and because SPARC is a secreted protein, at first, secreted SPARC protein was quantified using ELISA in the sera from 109 melanoma patients, 5 patients with large congenital melanocytic nevus, 61 age-matched healthy donors and 13 disease-free patients after undergoing a surgical removal. We also quantified two other known tumor markers for melanoma, GPC3 and 5-S-cysteinyldopa (5-S-CD), in the same serum samples, and compared these markers for their diagnostic value. Because of the similarities in the binding peptide motifs shared between H2-Kd and HLA-A24 (A*2402), 4 peptides having these motifs were synthesized. Then, we investigated whether the H2-Kd-restricted SPARC peptides could induce SPARC reactive cytotoxic T lymphocytes (CTLs) or not. In addition, we investigated the effect of the pre-immunization of BM-DC pulsed with the SPARC epitope peptides on a growth of SPARC-expressing tumor cells implanted in BALB/c mouse. |
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| 書誌情報 |
発行年 2007-03-27 |
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| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| 日本十進分類法 | ||||||||||||
| 主題 | 377.5 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 熊本大学 | |||||||||||
| 言語 | ja | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士（医学）(ja) | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関名 | 熊本大学 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2007-03-27 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第1579号 | |||||||||||
| 学位番号 | ||||||||||||
| 値 | 甲博医第1579号 | |||||||||||
