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Differentiation of mouse and human ES cells into hepatic lineages
http://hdl.handle.net/2298/11006
http://hdl.handle.net/2298/110069ade93fc-a3a0-46f1-8a93-0477c64772b7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Shiraki+GTC2008.pdf (1.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-12-06 | |||||
| タイトル | ||||||
| タイトル | Differentiation of mouse and human ES cells into hepatic lineages | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
白木, 伸明
× 白木, 伸明× 梅田, 香穂子× Sakashita, Naomi× 竹屋, 元裕× 粂, 和彦× 粂, 昭苑 |
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| 別言語の著者 |
粂, 昭苑
× 粂, 昭苑× 粂, 和彦 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | We recently reported a novel method to induce embryonic stem (ES) cells differentiate into an endodermal fate, especially pancreatic, using a supporting cell line. Here we describe the modified culture condition with the addition and withdrawal of secreted growth factors could induce ES cells to selectively differentiate into a hepatic fate efficiently. The signaling of BMP and FGF that have been implicated in hepatic differentiation during normal embryonic development are shown to play pivotal roles in generating hepatic cells from the definitive endoderm derived from ES cells. Moreover, the expression of AFP, Albumin or a biliary molecular marker appeared sequentially thus suggested the differentiation of ES cells recapitulated normal developmental processes of liver. The ES cell-derived differentiated cells showed evidence of glycogen storage, secreted Albumin, exhibited drug metabolism activities and expressed a set of cytochrome or drug conjugate enzymes, drug transporters specifically expressed in mature hepatocytes. With the same procedure, human ES cells also gave rise to cells with mature hepatocytes’ characteristics. In conclusion, this novel procedure for hepatic differentiation will be useful for elucidation of molecular mechanisms of hepatic fate decision at gut regionalization, and could represent an attractive approach for a surrogate cell source for pharmaceutical studies such as toxicology. | |||||
| 書誌情報 |
Genes to cells 巻 13, 号 7, p. 731-746, 発行年 2008-07 |
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| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11623816 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1111/j.1365-2443.2008.01201.x | |||||
| 権利 | ||||||
| 権利情報 | © 2008 by the Molecular Biology Society of Japan/Blackwell Publishing | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 13652443 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text/plain | |||||
| 形態 | ||||||
| 値 | 1373943 bytes | |||||
| 形態 | ||||||
| 値 | 58272 bytes | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 460 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | Differentiation of mouse and human embryonic stem cells into hepatic lineages | |||||
| 出版者 | ||||||
| 出版者 | Blackwell Publishing | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 雑誌掲載論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Journal Article | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 01 | |||||
