@article{oai:kumadai.repo.nii.ac.jp:00021579,
 author = {澤, 智裕 and Sawa, Tomohiro and 岡本, 竜哉 and Okamoto, Tatsuya and 芥, 照夫 and Akuta, Teruo and 徳冨, 芳子 and Tokutomi, Yoshiko and 光山, 勝慶 and Kim-Mitsuyama, Shokei and 藤井, 重元 and Fujii, Shigemoto and 赤池, 孝章 and 澤, 智裕 and Sawa, Tomohiro and Zaki, Mohammad Hasan and ザキ, モハマド ハサン and 岡本, 竜哉 and Okamoto, Tatsuya and 芥, 照夫 and Akuta, Teruo and 徳冨, 芳子 and Tokutomi, Yoshiko and 光山, 勝慶 and Kim-Mitsuyama, Shokei and Ihara, Hideshi and 居原, 秀 and 小林, 章 and Kobayashi, Akira and Yamamoto, Masayuki and ヤマモト, マサユキ and 藤井, 重元 and Fujii, Shigemoto and Arimoto, Hirokazu and アリモト, ヒデカズ and 赤池, 孝章 and Akaike, Takaaki},
 issue = {11},
 journal = {Nature Chemical Biology},
 month = {Nov},
 note = {application/pdf, application/pdf, 論文(Article), The signaling pathway of nitric oxide (NO) depends mainly on guanosine 3′,5′-cyclic monophosphate (cGMP, 1). Here we report the formation and chemical biology of a nitrated derivative of cGMP, 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP, 2), in NO-mediated signal transduction. Immunocytochemistry demonstrated marked 8-nitro-cGMP production in various cultured cells in an NO-dependent manner. This finding was confirmed by HPLC plus electrochemical detection and tandem mass spectrometry. 8-Nitro-cGMP activated cGMP-dependent protein kinase and showed unique redox-active properties independent of cGMP activity. Formation of protein Cys-cGMP adducts by 8-nitro-cGMP was identified as a new post-translational modification, which we call protein S-guanylation. 8-Nitro-cGMP seems to regulate the redox-sensor signaling protein Keap1, via S-guanylation of the highly nucleophilic cysteine sulfhydryls of Keap1. This study reveals 8-nitro-cGMP to be a second messenger of NO and sheds light on new areas of the physiology and chemical biology of signal transduction by NO.},
 pages = {727--735},
 title = {Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate},
 volume = {3},
 year = {2007},
 yomi = {サワ, トモヒロ and オカモト, タツヤ and アクタ, テルオ and トクトミ, ヨシコ and ミツヤマ, ショウケイ and フジイ, シゲモト and サワ, トモヒロ and オカモト, タツヤ and アクタ, テルオ and トクトミ, ヨシコ and ミツヤマ, ショウケイ and イハラ, ヒデシ and コバヤシ, アキラ and フジイ, シゲモト and アカイケ, タカアキ}
}