{"created":"2023-06-19T09:10:22.067775+00:00","id":21866,"links":{},"metadata":{"_buckets":{"deposit":"ec0a84ff-60c7-4c26-9591-727e9d405632"},"_deposit":{"created_by":1,"id":"21866","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"21866"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00021866","sets":["415:416"]},"author_link":["94659","94615","94671","94617","94669","94667","94670","94658","94668","94310"],"item_16_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"1242","bibliographicPageStart":"1232","bibliographicVolumeNumber":"97","bibliographic_titles":[{"bibliographic_title":"Journal of Neurochemistry"}]}]},"item_16_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"香月, 博志"}],"nameIdentifiers":[{"nameIdentifier":"94667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大川原, 賦"}],"nameIdentifiers":[{"nameIdentifier":"94668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柴田, 治樹"}],"nameIdentifiers":[{"nameIdentifier":"94669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久米, 利明"}],"nameIdentifiers":[{"nameIdentifier":"94670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"赤池, 昭紀"}],"nameIdentifiers":[{"nameIdentifier":"94671","nameIdentifierScheme":"WEKO"}]}]},"item_16_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_16_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_16_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Activated microglia are considered to play important roles in degenerative processes of midbrain dopaminergic neurons. Here we examined mechanisms of neurotoxicity of thrombin, a protease known to trigger microglial activation, in organotypic midbrain slice cultures. Thrombin induced a progressive decline in the number of dopaminergic neurons, an increase in nitric oxide (NO) production, and whole tissue injury indicated by lactate dehydrogenase release and propidium iodide uptake. Microglia expressed inducible NO synthase (iNOS) in response to thrombin, and inhibition of iNOS rescued dopaminergic neurons without affecting whole tissue injury. Inhibitors of mitogen-activated protein kinases (MAPKs) such as extracellular signal-regulated kinase (ERK), p38 MAPK and c-Jun N-terminal kinase (JNK) attenuated thrombin-induced iNOS induction and dopaminergic cell death. Whole tissue injury was also attenuated by inhibition of ERK and p38 MAPK. Moreover, depletion of resident microglia from midbrain slices abrogated thrombin-induced NO production and dopaminergic cell death, but did not inhibit tissue injury. Finally, antioxidative drugs prevented thrombin-induced dopaminergic cell death without affecting whole tissue injury. Hence, NO production resulting from MAPK-dependent microglial iNOS induction is a crucial event in thrombin-induced dopaminergic neurodegeneration, whereas damage of other midbrain cells is MAPK-dependent but is NO-independent.","subitem_description_type":"Other"}]},"item_16_description_77":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_description":"http://www.blackwellpublishing.com/jnc_enhanced/","subitem_description_type":"Other"}]},"item_16_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Blackwell Science"}]},"item_16_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/j.1471-4159.2006.03752.x","subitem_relation_type_select":"DOI"}}]},"item_16_relation_60":{"attribute_name":"部分である","attribute_value_mlt":[{"subitem_relation_type":"isPartOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/2298/9868","subitem_relation_type_select":"URI"}}]},"item_16_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00223042","subitem_source_identifier_type":"ISSN"}]},"item_16_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00703243","subitem_source_identifier_type":"NCID"}]},"item_16_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491.17","subitem_subject_scheme":"NDC"}]},"item_16_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"1210829 bytes"}]},"item_16_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"雑誌掲載論文"}]},"item_16_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Journal Article"}]},"item_16_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_16_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"01"}]},"item_16_text_78":{"attribute_name":"コメント","attribute_value_mlt":[{"subitem_text_value":"平成18～19年度科学研究費補助金(基盤研究(C))研究成果報告書 課題番号:18590052『ミクログリアの活性化を介する黒質線条体系ニューロン変性の機序とその防御』pp.33-43に掲載"}]},"item_16_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"香月, 博志","creatorNameLang":"ja"},{"creatorName":"カツキ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Katsuki, Hiroshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"香月","familyNameLang":"ja"},{"familyName":"カツキ","familyNameLang":"ja-Kana"},{"familyName":"Katsuki","familyNameLang":"en"}],"givenNames":[{"givenName":"博志","givenNameLang":"ja"},{"givenName":"ヒロシ","givenNameLang":"ja-Kana"},{"givenName":"Hiroshi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"94310","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okawara, Mitsugu"}],"nameIdentifiers":[{"nameIdentifier":"94658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibata, Haruki"}],"nameIdentifiers":[{"nameIdentifier":"94659","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"久米, 利明","creatorNameLang":"ja"},{"creatorName":"クメ, トシアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kume, Toshiaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"久米","familyNameLang":"ja"},{"familyName":"クメ","familyNameLang":"ja-Kana"},{"familyName":"Kume","familyNameLang":"en"}],"givenNames":[{"givenName":"利明","givenNameLang":"ja"},{"givenName":"トシアキ","givenNameLang":"ja-Kana"},{"givenName":"Toshiaki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"94615","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"赤池, 昭紀","creatorNameLang":"ja"},{"creatorName":"アカイケ, アキノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Akaike, Akinori","creatorNameLang":"en"}],"familyNames":[{"familyName":"赤池","familyNameLang":"ja"},{"familyName":"アカイケ","familyNameLang":"ja-Kana"},{"familyName":"Akaike","familyNameLang":"en"}],"givenNames":[{"givenName":"昭紀","givenNameLang":"ja"},{"givenName":"アキノリ","givenNameLang":"ja-Kana"},{"givenName":"Akinori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"94617","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"J Neu0097-1232.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"J Neu0097-1232.pdf","url":"https://kumadai.repo.nii.ac.jp/record/21866/files/J Neu0097-1232.pdf"},"version_id":"38c188ed-7a3d-471b-9bb5-5dd8aeecb6a2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Dopamine neuron","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammation","subitem_subject_scheme":"Other"},{"subitem_subject":"Mitogen-activated protein kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"Neurodegeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"Parkinson's disease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nitric oxide-producing microglia mediate thrombin-induced degeneration of dopaminergic neurons in rat midbrain slice culture","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nitric oxide-producing microglia mediate thrombin-induced degeneration of dopaminergic neurons in rat midbrain slice culture"}]},"item_type_id":"16","owner":"1","path":["416"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-12-02"},"publish_date":"2008-12-02","publish_status":"0","recid":"21866","relation_version_is_last":true,"title":["Nitric oxide-producing microglia mediate thrombin-induced degeneration of dopaminergic neurons in rat midbrain slice culture"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-09-26T01:17:14.356872+00:00"}