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Application of Promoter Modified Electrodes to Bioelectrochemical Measurements on the Effects of Origin and Modification of Lysine Residues of Cytochrome c
http://hdl.handle.net/2298/10570
http://hdl.handle.net/2298/10570595e6689-e15b-475a-8de8-4e1ac5b5df35
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1992Anal Sci-Cytc-Modified-CytOx.pdf (527.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2009-01-30 | |||||
| タイトル | ||||||
| タイトル | Application of Promoter Modified Electrodes to Bioelectrochemical Measurements on the Effects of Origin and Modification of Lysine Residues of Cytochrome c | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Cytochrome c, cytochrome c oxidase, promoter modified electrode, electron transfer reaction, respiratory chain, molecular recognition | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Tominaga, Masato
× Tominaga, Masato× Hayashi, Kuniaki× Taniguchi, Isao |
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| 別言語の著者 |
冨永, 昌人
× 冨永, 昌人× 谷口, 功 |
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| 内容記述 | ||||||
| 内容記述 | Using promoter-modified electrodes, electron-transfer reactions of cytochrome c at an electrode and with cytochrome c oxidase were examined. The electrochemical behavior of cytochromes c from horse, bovine, chicken and tuna hearts at an electrode was similar to each other. Little difference was observed for the reactions between cytochromes c of various origins and cytochrome c oxidase from bovine in a phosphate buffer solution. Also, when a few (less than three) lysine residues of cytochrome c were treated with 4-chloro-3, 5-dinitrobenzoic acid (CDNP) or 2, 4-dinitrofluorobenzene (DNP), the electrode reaction of the modified (especially, mono-substituted) cytochrome c still showed no significant difference from that of the native one. On the other hand, the electron-transfer reaction between CDNP- or DNP-substituted cytochrome c at lysine 13 and/or 72 and cytochrome c oxidase was greatly affected, even when only one or two lysine residues of cytochrome c were modified. Since the cytochrome c molecules of different origins examined have about 3-19 differences in their amino acid sequences, but most lysine residues remain invariant, the present results indicate that lysine residues of cytochrome c play an important role in the interaction with cytochrome c oxidase; however, the promoter modified electrode surface does not recognize cytochrome c molecule very rigorously. The present study also shows that electrochemical techniques with the aid of functional electrodes are applicable as useful, convenient methods to analyze the biological reactions of proteins. | |||||
| 書誌情報 |
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 巻 8, 号 6, p. 829-836, 発行年 1992-12 |
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| ISSN | ||||||
| 収録物識別子 | 13482246 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10500785 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 関連識別子 | 10.2116/analsci.8.829 | |||||
| 権利 | ||||||
| 権利情報 | ©Japan Society for Analytical Chemistry | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 09106340 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 527555 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 464 | |||||
| 出版者 | ||||||
| 出版者 | Japan Society for Analytical Chemistry | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 01 | ||||||
| 著作の一部 | ||||||
| 関連名称 | http://dx.doi.org/10.2116/analsci.8.829 | |||||
| コメント | ||||||
| 社団法人 日本分析化学会（http://www.jsac.or.jp/） | ||||||
