{"created":"2023-06-19T09:10:45.040206+00:00","id":22290,"links":{},"metadata":{"_buckets":{"deposit":"80b7a06c-acaf-4942-9734-f3211f8d7ced"},"_deposit":{"created_by":1,"id":"22290","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"22290"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00022290","sets":["343:344:345:346"]},"author_link":["96894","96893","96895"],"item_14_alternative_title_22":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-βgeo cassette"}]},"item_14_alternative_title_23":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"カヘンガタ イデンシ  トラップ ベクター pU-17 オ モチイタ トラップ ライン ノ ジュリツ ト カイセキ"}]},"item_14_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-11","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_14_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Taniwaki, Takuya"}],"nameIdentifiers":[{"nameIdentifier":"96895","nameIdentifierScheme":"WEKO"}]}]},"item_14_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"},{"subitem_description":"application/pdf","subitem_description_type":"Other"},{"subitem_description":"text/plain","subitem_description_type":"Other"}]},"item_14_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"学位論文(Thesis)","subitem_description_type":"Other"}]},"item_14_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"We have developed a new exchangeable gene trap vector, pU-17, carrying the intron-lox71-splicing acceptor (SA)-β\ngeo-loxP-pA-lox2272-pSP73-lox511. The SA contains three stop codons in-frame with the ATG of β galactosidase/neomycin-resistance\nfusion gene(β geo) that can function in promoter trapping. We found that the trap vector was highly selective for integrations in the introns adjacent to the exon containing the start codon. Furthermore, by using the Cre-mutant lox system, we successfully replaced the β geo gene with the enhanced green fluorescent protein (EGFP) gene, established mouse lines with the replaced clones, removed the selection marker gene by mating with Flp-deleter mice, and confirmed that the replaced EGFP gene was expressed in the same pattern as the β geo gene. Thus, using this pU-17 trap vector, we can initially carry out random mutagenesis, and then replace the β geo gene with any gene of interest to be expressed under the control of the trapped promoter through Cre-mediated recombination.","subitem_description_type":"Other"}]},"item_14_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"熊本大学"}]},"item_14_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"377.5","subitem_subject_scheme":"NDC"}]},"item_14_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"2260236 bytes"},{"subitem_text_value":"60911 bytes"},{"subitem_text_value":"45827 bytes"}]},"item_14_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"博士論文"}]},"item_14_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Thesis or Dissertation"}]},"item_14_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_14_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"03"}]},"item_14_text_81":{"attribute_name":"学位番号","attribute_value_mlt":[{"subitem_text_value":"乙博医第907号"}]},"item_14_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"谷脇, 琢也"}],"nameIdentifiers":[{"nameIdentifier":"96893","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"32-0907.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"32-0907.pdf","url":"https://kumadai.repo.nii.ac.jp/record/22290/files/32-0907.pdf"},"version_id":"fc521ec1-f2e4-4eac-8537-e5fcce1f59df"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"エレクトロポレーション","subitem_subject_scheme":"Other"},{"subitem_subject":"genomic DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA解析","subitem_subject_scheme":"Other"},{"subitem_subject":"キメラマウス","subitem_subject_scheme":"Other"},{"subitem_subject":"splice acceptor(SA)","subitem_subject_scheme":"Other"},{"subitem_subject":"Cre-mutant lox system","subitem_subject_scheme":"Other"},{"subitem_subject":"enchanged green fluorescent protein（EGFP）遺伝","subitem_subject_scheme":"Other"},{"subitem_subject":"internal ribosomal entry site(IRES)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"可変型遺伝子トラップベクターpU-17を用いたトラップラインの樹立と解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"可変型遺伝子トラップベクターpU-17を用いたトラップラインの樹立と解析"}]},"item_type_id":"14","owner":"1","path":["346"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-08-12"},"publish_date":"2014-08-12","publish_status":"0","recid":"22290","relation_version_is_last":true,"title":["可変型遺伝子トラップベクターpU-17を用いたトラップラインの樹立と解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T18:54:32.423863+00:00"}