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Limulus amebocyte Lysate assay for endotoxins by an adsorption Method with Polycation-immobilized Cellulose Beads
http://hdl.handle.net/2298/15190
http://hdl.handle.net/2298/151902ba7348b-ba4c-49d8-8a81-e40914bb87d7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AnaSci2010_26_291.pdf (961.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2010-07-02 | |||||
| タイトル | ||||||
| タイトル | Limulus amebocyte Lysate assay for endotoxins by an adsorption Method with Polycation-immobilized Cellulose Beads | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
坂田, 眞砂代
× 坂田, 眞砂代× Inoue, Tomofumi× Todokoro, Masami× 國武, 雅司 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | To assay lipopolysaccharides (LPSs) in solutions containing Limulus amebocyte lysate (LAL)-inhibiting or LAL-enhancing compounds, we developed a selective endotoxin (LPS) assay using poly(ε-lysine)-immobilized cellulose beads (PL-Cellufine) and LAL. The PL-Cellufine can adsorb LPSs in a solution containing certain compounds (NaCl, proteins and amino acids) at an ionic strength of μ = 0.05 – 0.4 at neutral pH. The LPSs adsorbed on the PL-Cellufine were separated from the compounds by centrifugation and then the PL-Cellufine was suspended in LPS-free water. The LPS activities of the suspension are directly assayed by a turbidimetric time assay with the LAL reagent. The accuracy of the adsorption method was high compared with those of common solution methods. As for the common method, the apparent recovery of LPS from the compounds was 40 – 95%. This suggests that these compounds inhibit the LAL procedure. By contrast, the adsorption method showed good LPS recovery (88 – 120%) in all cases, without being inhibited or enhanced by the compounds. | |||||
| 書誌情報 |
ANALYTICAL SCIENCES 巻 26, 号 3, p. 291-296, 発行年 2010-03-10 |
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| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 関連識別子 | https://doi.org/10.2116/analsci.26.291 | |||||
| 権利 | ||||||
| 権利情報 | © The Japan Society for Analytical Chemistry | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 1348-2246 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 値 | 961831 bytes | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 433 | |||||
| 出版者 | ||||||
| 出版者 | The Japan Society for Analytical Chemistry | |||||
| 別言語の出版者 | ||||||
| 値 | 社団法人 日本分析化学会 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 雑誌掲載論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Journal Article | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 01 | |||||
| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.jstage.jst.go.jp/article/analsci/26/3/291/_pdf/-char/ja/ | |||||
