@article{oai:kumadai.repo.nii.ac.jp:00023779,
 author = {香月, 博志 and 黒須, 真介 and 道永, 昌太郎 and 久恒, 昭哲 and 礒濱, 洋一郎 and 泉, 安彦 and 久米, 利明 and 赤池, 昭紀 and 香月, 博志 and Katsuki, Hiroshi and Kurosu, Shinsuke and Michinaga, Shotaro and Hisatsune, Akinori and Isohama, Yoichiro and Izumi, Yasuhiko and 久米, 利明 and Kume, Toshiaki and 赤池, 昭紀 and Akaike, Akinori},
 issue = {6},
 journal = {Peptides},
 month = {Jun},
 note = {application/pdf, 論文(Article), A hypothalamic neuropeptide orexin (hypocretin) is a critical regulator of physiological processes including sleep/wakefulness and feeding. Using organotypic slice culture of rat hypothalamus, we found that exposure to elevated extracellular concentration of K+ (+10-30 mM) for 24-72 h led to a substantial decrease in the number of neurons immunoreactive for orexin and a co-existing neuropeptide dynorphin-A. In contrast, the same treatment affected neither the number of melanin-concentrating hormone-immunoreactive neurons nor the number of total neurons. A substantial decrease of orexin-immunoreactive neurons was also induced by 72 h treatment with 1-10 μM veratridine, a Na+ channel activator. The effect of elevated K+ was only partially reversible, and that of veratridine was virtually irreversible, although the decrease in orexin immunoreactivity was not associated with signs of cell damage assessed by propidium iodide uptake and Hoechst 33342 nuclear staining. In addition, the level of preproorexin mRNA did not decrease during treatment with elevated K+ or veratridine. After treatment with elevated K+ and veratridine, c-Fos immunoreactivity appeared in orexin-immunoreactive neurons but not in melanin-concentrating hormone-immunoreactive neurons, suggesting selective excitation of orexin neurons. However, the amount of orexin released extracellularly was paradoxically decreased by treatment with elevated K+ and veratridine. Overall, these characteristics of orexin neurons may be taken into consideration to understand the behaviors of these neurons under physiological and pathophysiological conditions., http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T0M-4YN5P64-1&_user=4545557&_coverDate=06%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000008298&_version=1&_urlVersion=0&_userid=4545557&md5=88271472653e993e41bb078e216cb9ec},
 pages = {1131--1138},
 title = {Depolarizing stimuli cause persistent and selective loss of orexin in rat hypothalamic slice culture},
 volume = {31},
 year = {2010},
 yomi = {カツキ, ヒロシ and クメ, トシアキ and アカイケ, アキノリ}
}