{"created":"2023-06-19T09:11:57.308941+00:00","id":23781,"links":{},"metadata":{"_buckets":{"deposit":"12848124-5387-463e-9d0a-a420ff640787"},"_deposit":{"created_by":1,"id":"23781","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"23781"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00023781","sets":["413:443"]},"author_link":["104583","104582","104573","104571","104580","104584","104569","104581","104572","104566","104567","104568","94588","104570"],"item_16_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"981","bibliographicPageStart":"966","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"Apoptosis"}]}]},"item_16_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西浦, 弘志"}],"nameIdentifiers":[{"nameIdentifier":"104580","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"斎田, 和孝"}],"nameIdentifiers":[{"nameIdentifier":"104581","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原田, 幸一"}],"nameIdentifiers":[{"nameIdentifier":"104582","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西野, 憲和"}],"nameIdentifiers":[{"nameIdentifier":"104583","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山本, 哲郎"}],"nameIdentifiers":[{"nameIdentifier":"104584","nameIdentifierScheme":"WEKO"}]}]},"item_16_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_16_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_16_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"We have demonstrated that an alternative C5a receptor (C5aR) ligand, the homodimer of ribosomal protein S19 (RP S19), contains a unique C-terminus (I134–H145) that is distinct from the moieties involved in the C5a–C5aR interaction. To examine the role of I134–H145 in the ligand–C5aR interaction, we connected this peptide to the C-terminus of C5a (C5a/RP S19) and found that it endowed the second binding moiety of RP S19 (L131DR) with a relatively higher binding affinity to the C5aR on a human mast cell line, HMC-1. In contrast to the C5aR, the second C5aR C5L2 worked as a decoy receptor. As a result, the mitogen-activated protein kinase (MAPK) downstream of the Gi protein exchanged extracellular-signal regulated kinase for p38MAPK. This alternative p38MAPK activation could be pharmacologically suppressed not only by the downregulation of phosphoinositide 3-kinase (PI3K) by LY294002, but also by the over-activation of protein kinase C by phorbol 12-myristate 13-acetate. The activation was reproduced upon C5a–C5aR interaction by a simultaneous suppression of PI3K and phospholipase C with LY294002 and U73122 at low concentrations. Moreover, p38MAPK phosphorylation upstream of the pertussis toxin-dependent extracellular Ca2+ entry was also suppressed by high concentrations of MgCl2, which blocks melastatin-type transient receptor potential Ca2+ channels (TRPMs). The active conformation of C5aR upon the ligation by C5a, at least on HMC-1 cells, is changed by the additional interaction of the I134–H145 peptide, which seems to guide the alternative activation of p38MAPK. This activation is then amplified by a novel positive feedback loop between p38MAPK and TRPM.\nElectronic supplementary material  The online version of this article (doi:10.1007/s10495-010-0511-y) contains supplementary material, which is available to authorized users.","subitem_description_type":"Other"}]},"item_16_description_77":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_description":"http://www.springerlink.com/content/w47x84142mw45723/","subitem_description_type":"Other"}]},"item_16_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Netherlands"}]},"item_16_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"20473571","subitem_relation_type_select":"PMID"}}]},"item_16_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10495-010-0511-y","subitem_relation_type_select":"DOI"}}]},"item_16_relation_16":{"attribute_name":"情報源(ISSN)","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"13608185"}]}]},"item_16_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Springer Science+Business Media"}]},"item_16_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_16_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"601180 bytes"}]},"item_16_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"雑誌掲載論文"}]},"item_16_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Journal Article"}]},"item_16_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_16_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"01"}]},"item_16_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishiura, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"104566","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokita, Kazutaka"}],"nameIdentifiers":[{"nameIdentifier":"104567","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Ying"}],"nameIdentifiers":[{"nameIdentifier":"104568","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Harada, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"104569","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Trent,  M. Woodruff"}],"nameIdentifiers":[{"nameIdentifier":"104570","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Stephen, M. Taylor"}],"nameIdentifiers":[{"nameIdentifier":"104571","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tienabe, Kipassa Nsiama"}],"nameIdentifiers":[{"nameIdentifier":"104572","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishino, Norikazu"}],"nameIdentifiers":[{"nameIdentifier":"104573","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"山本, 哲郎","creatorNameLang":"ja"},{"creatorName":"ヤマモト, テツロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamamoto, Tetsuro","creatorNameLang":"en"}],"familyNames":[{"familyName":"山本","familyNameLang":"ja"},{"familyName":"ヤマモト","familyNameLang":"ja-Kana"},{"familyName":"Yamamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"哲郎","givenNameLang":"ja"},{"givenName":"テツロウ","givenNameLang":"ja-Kana"},{"givenName":"Tetsuro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"94588","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"Apoptosis_HIRO minor revised paper_r.pdf","filesize":[{"value":"601.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Apoptosis_HIRO minor revised paper_r.pdf","url":"https://kumadai.repo.nii.ac.jp/record/23781/files/Apoptosis_HIRO minor revised paper_r.pdf"},"version_id":"c041688a-c93f-4f37-b61f-6fdd4e93694d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"C5a receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"Gi protein","subitem_subject_scheme":"Other"},{"subitem_subject":"HMC-1 cells","subitem_subject_scheme":"Other"},{"subitem_subject":"p38MAPK","subitem_subject_scheme":"Other"},{"subitem_subject":"PI3K","subitem_subject_scheme":"Other"},{"subitem_subject":"Ribosomal protein S19","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The role of the ribosomal protein S19 C-terminus in Gi protein-dependent alternative activation of p38 MAP kinase via the C5a receptor in HMC-1 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The role of the ribosomal protein S19 C-terminus in Gi protein-dependent alternative activation of p38 MAP kinase via the C5a receptor in HMC-1 cells"}]},"item_type_id":"16","owner":"1","path":["443"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-08-01"},"publish_date":"2011-08-01","publish_status":"0","recid":"23781","relation_version_is_last":true,"title":["The role of the ribosomal protein S19 C-terminus in Gi protein-dependent alternative activation of p38 MAP kinase via the C5a receptor in HMC-1 cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-09-14T01:59:05.893877+00:00"}