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Complete chromatographic separation of steroids, including 17α and 17β-estradiols, using a carbazole-based polymeric organic phase in both reversed and normal-phase HPLC
http://hdl.handle.net/2298/18712
http://hdl.handle.net/2298/18712040ae3c8-f4d8-492a-a645-5a9471e31ef7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ABC_397_2_623-629.pdf (1.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-05-06 | |||||
| タイトル | ||||||
| タイトル | Complete chromatographic separation of steroids, including 17α and 17β-estradiols, using a carbazole-based polymeric organic phase in both reversed and normal-phase HPLC | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Carbazole-based polymer, Separation, Steroids, Solvent effect | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Mallik, Abul K.
× Mallik, Abul K.× 新郷, かおり× Usha, Ghimire Gautam× 澤田, 剛× 高藤, 誠× 伊原, 博隆 |
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| 別言語の著者 |
新郷, かおり
× 新郷, かおり× 澤田, 剛× 高藤, 誠× 伊原, 博隆 |
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| 内容記述 | ||||||
| 内容記述 | Poly(2-N-carbazolylethyl acrylate) with terminal trimethoxysilyl groups was prepared as an 2 organic phase and immobilized onto silica. The retention behavior of the column packed with this carbazole-based polymer-immobilized silica (Sil-CEA) was investigated by using various estrogenic steroids and corticoids in both reversed phase and normal phase liquid chromatographies. As a result, the complete separation was confirmed for eight kinds of steroids with Sil-CEA. The most specific separation with Sil-CEA can be emphasized by the high separation factor (e.g., α = 1.39 in methanol-water (7:3, v/v) at 35 °C) for 17α- and 17β-estradioles, that are one of the most difficult isomers in chromatographic separation, while two kinds of commercially available polymeric ODSs as references shows only α = 1.01 at the same condition. Since the excellent separation and retention order with Sil-CEA was maintained even in a normal phase mobile phase such as a hexane-2-propanol mixture, it is estimated that the CEA phase has multiple interaction mechanisms through stronger interactions such as dipole-dipole, carbonyl-π and hydrogen bonding interactions than a hydrophobic effect expected in ODS. | |||||
| 書誌情報 |
Analytical and Bioanalytical Chemistry 巻 397, 号 2, p. 623-629, 発行年 2010-05 |
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| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1007/s00216-010-3601-3 | |||||
| 権利 | ||||||
| 権利情報 | ©Springer-Verlag 2010 | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 1618-2642 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 1943262 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 570 | |||||
| 出版者 | ||||||
| 出版者 | Springer Berlin / Heidelberg | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
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| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
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| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.springerlink.com/content/k4003417ug062622/ | |||||
