{"created":"2023-06-19T09:12:43.719233+00:00","id":24600,"links":{},"metadata":{"_buckets":{"deposit":"c849843b-a48a-4531-8f25-1f55dcaf7cdd"},"_deposit":{"created_by":1,"id":"24600","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"24600"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00024600","sets":["413:429"]},"author_link":["109739","109741","109740"],"item_18_alternative_title_22":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Development of Novel Therapy by miRNA Targeting for Gastroenterological Cancer"}]},"item_18_alternative_title_23":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"miRNA オ ターゲット ト シタ ショウカキ ガン ニ タイスル アラタナ チリョウ センリャク ノ カイハツ"}]},"item_18_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06-23","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_18_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Baba, Hideo"}],"nameIdentifiers":[{"nameIdentifier":"109741","nameIdentifierScheme":"WEKO"}]}]},"item_18_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf"}]},"item_18_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_18_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"対象20症例中18症例において、正常上皮と比較して癌組織でmicroRNA-21(miR-21)が高発現していた。特にリンパ節転移陽性例、または静脈侵襲陽性例では有意に発現が高かった。また、7種類の食道癌細胞株はいずれもmiR-21が高発現していた。Anti-miR-21-inhibitorをトランスフェクトしてmiR-21の発現を抑制した細胞は、増殖能ならびに浸潤能が有意に抑制された。食道癌細胞株におけるProgrammed Cell Death 4(PDCD4)蛋白の発現とmiR-21の発現は有意に逆相関していた。Anti-miR-21-inhibitorをトランスフェクトした細胞株では、PDCD4のmRNAは変化しなかったが、蛋白レベルではPDCD4 の発現が有意に増加した。また、PDCD4mRNAの3'-非翻訳領域を導入したルシフェラーゼ活性が有意に増加した。食道扁平上皮癌120例でPDCD4免疫染色を行うと、正常上皮と比較して食道扁平上皮癌ではPDCD4発現が減弱しており、PDCD4陰性例は陽性症例よりも進行症例が多く、予後不良であった。miR-21はPDCD4などの癌抑制遺伝子を翻訳レベルで抑制していると考えられた。miR-21が食道扁平上皮癌治療における新たなターゲットとなる可能性がある。","subitem_description_type":"Other"},{"subitem_description":"Among 20 paired samples, 18 cancer tissues overexpressed miR-21 compared with matched normal epitheliums. Especially, patients with lymph node metastasis or venous invasion showed high expression of miR-21 significantly. All 7 ESCC cell lines also overexpressed miR-21 and anti-miR-21 transfected ESCC cells showed significant reduction in cellular proliferation and\ninvasion. The PDCD4 protein in ESCC cells have inverse correlation with miR-21 expression significantly. Anti-miR-21 transfected ESCC cells increased PDCD4 protein without differences of PDCD4-mRNA and increased a luciferase-reporter activity containing the PDCD4-3’-UTR region. Among 120 ESCC samples, immunohistchmeistry study revealed that patients expressed PDCD4 protein prolonged survival compared with negative. Our results suggest miR-21 plays a pivotal role in progression of ESCC, and it might serve as a novel therapeutic target.","subitem_description_type":"Other"}]},"item_18_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"熊本大学"}]},"item_18_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"493.4","subitem_subject_scheme":"NDC"}]},"item_18_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"85032 bytes"}]},"item_18_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"研究報告書"}]},"item_18_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Research Paper"}]},"item_18_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_18_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"06"}]},"item_18_text_78":{"attribute_name":"コメント","attribute_value_mlt":[{"subitem_text_value":"平成19～21年度科学研究費補助金(基盤研究(B))研究成果報告書　課題番号：19390338"}]},"item_18_text_80":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_text_value":"19390338"}]},"item_18_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"馬場, 秀夫"}],"nameIdentifiers":[{"nameIdentifier":"109739","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"KaB19390338s.pdf","filesize":[{"value":"85.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KaB19390338s.pdf","url":"https://kumadai.repo.nii.ac.jp/record/24600/files/KaB19390338s.pdf"},"version_id":"2a70469c-7726-4448-9f3b-d982042f3178"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"実験外科学","subitem_subject_scheme":"Other"},{"subitem_subject":"消化器癌","subitem_subject_scheme":"Other"},{"subitem_subject":"分子生物学","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"miRNAをターゲットとした消化器癌に対する新たな治療戦略の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"miRNAをターゲットとした消化器癌に対する新たな治療戦略の開発"}]},"item_type_id":"18","owner":"1","path":["429"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-03-07"},"publish_date":"2011-03-07","publish_status":"0","recid":"24600","relation_version_is_last":true,"title":["miRNAをターゲットとした消化器癌に対する新たな治療戦略の開発"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T18:23:29.262448+00:00"}