@article{oai:kumadai.repo.nii.ac.jp:00024667,
 author = {異島, 優 and 廣山, 秀一 and 丸山, 徹 and 澤, 智裕 and 赤池, 孝章 and 甲斐, 俊哉 and 小田切, 優樹 and Ishima, Yu and Hiroyama, Shuichi and Ulrich, Kragh-Hansen and Maruyama, Toru and 澤, 智裕 and Sawa, Tomohiro and 赤池, 孝章 and Akaike, Takaaki and Kai, Toshiya and Otagiri, Masaki},
 issue = {2},
 journal = {Nitric Oxide},
 month = {Sep},
 note = {application/pdf, application/pdf, application/pdf, 論文(Article), S-Nitrosated human serum albumin (SNO-HSA) is a large molecular weight nitric oxide carrier in human plasma, and because of its many beneficial effects in different tests, it is currently under investigation as a cytoprotective agent. However, making SNO-HSA preparations is a complicated and time-consuming process. We found that binding of caprylic acid (CA) and N-acetyl-L-tryptophan (N-AcTrp) to defatted mercaptalbumin increased S-nitrosation by S-nitrosoglutathione (GS-NO) by making Cys-34 of HSA more accessible and by protecting it against oxidation, respectively. Fortunately, HSA solutions for clinical use contain high concentrations of CA and N-AcTrp as stabilizers. By making use of that fact it was possible to work-out a fast and simple procedure for producing SNO-HSA: Incubation of a commercial HSA formulation with GS-NO for only one minute results in S-nitrosation of HSA. The biological usefulness of such a preparation was tested in a rat ischemia-reperfusion liver injury model. Although our procedure for making SNO-HSA is fast and straightforward, the cytoprotective effect of the preparation was similar to, or better than, that of a preparation made in a more traditional way. The clinical development of SNO-HSA as a strong cytoprotective agent is under way using this method in collaboration with clinicians and industrial developers., http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WNT-5017HTP-1&_user=4545557&_coverDate=09%2F15%2F2010&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000008298&_version=1&_urlVersion=0&_userid=4545557&md5=50f652d134dd09a6f72481d07de2387d&searchtype=a},
 pages = {121--127},
 title = {One-step preparation of S-nitrosated human serum albumin with high biological activities},
 volume = {23},
 year = {2010},
 yomi = {サワ, トモヒロ and アカイケ, タカアキ}
}