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Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages
http://hdl.handle.net/2298/20639
http://hdl.handle.net/2298/206390b0b7175-e925-4bf7-b0e0-d4e7f9d28544
| 名前 / ファイル | ライセンス | アクション |
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JCS_123_Figures.pdf (2.3 MB)
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JCS_123_2733-2742.pdf (156.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-08-02 | |||||
| タイトル | ||||||
| タイトル | Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Embryonic stem cells, Endoderm, In vitro differentiation, Pancreatic differentiation, Synthesized basement membrane (sBM), Extracellular matrix | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Higuchi, Yuichiro
× Higuchi, Yuichiro× 白木, 伸明× Yamane, Keitaro× Qin, Zeng× Mochitate, Katsumi× 荒木, 喜美× Senokuchi, Takafumi× Yamagata, Kazuya× Hara, Manami× 粂, 和彦× Kume, Shoen |
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| 別言語の著者 |
樋口, 裕一郎
× 樋口, 裕一郎× 白木, 伸明× 山根, 恵太郎× 持立, 克身× 荒木, 喜美× 瀬ノ口, 隆文× 山縣, 和也× 粂, 和彦× 粂, 昭苑 |
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| 内容記述 | ||||||
| 内容記述 | We previously reported that embryonic stem (ES) cells cultured on M15 cells, a mesoderm-derived supportive cell line, were efficiently differentiated towards an endodermal fate, finally adopting the specific lineages of various digestive organs such as the pancreas and liver. We show here that the endoderm-inducing activity of M15 cells is in part mediated through the extracellular matrices, and that laminin α5 is one of the crucial components. In an attempt to establish a feeder-free ES-cell procedure for pancreatic differentiation, we used a synthesized basement membrane (sBM) substratum using an HEK293 cell line stably expressing laminin-511. On the sBM, mouse ES or induced pluripotent stem (iPS) cells sequentially differentiated into the definitive endoderm, pancreatic progenitor cells, and then insulin-expressing pancreatic β-cells in vitro. Knockdown of ES cells with integrin β1 (Itgb1) reduces differentiation towards pancreatic cells. Heparan sulfate proteoglycan 2 (HSPG2) knockdown and heparitinase treatment synergistically decreased the number of Pdx1-expressing cells. These findings indicate that components of the basement membrane have an important role in the differentiation of definitive endoderm lineages. This novel procedure will be useful for the study of pancreatic differentiation of ES or iPS cells and the generation of potential sources of surrogate cells for regenerative medicine. | |||||
| 書誌情報 |
Journal of Cell Science 巻 123, 号 16, 15, p. 2733-2742, 発行年 2010-08 |
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| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 20647375 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1242/jcs.066886 | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 00219533 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
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| 156257 bytes | ||||||
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| 2324202 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 460 | |||||
| 出版者 | ||||||
| 出版者 | Company of Biologists | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
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| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://jcs.biologists.org/content/123/16/2733 | |||||
