WEKO3
アイテム
Failure of Effector Function of Human CD8+ T Cells in NOD/SCID/JAK3−/− Immunodeficient Mice Transplanted with Human CD34+ Hematopoietic Stem Cells
http://hdl.handle.net/2298/20741
http://hdl.handle.net/2298/20741f2a8a431-be6a-4077-9a2e-2125a2dd917f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
journal.pone.0013109.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-08-08 | |||||
| タイトル | ||||||
| タイトル | Failure of Effector Function of Human CD8+ T Cells in NOD/SCID/JAK3−/− Immunodeficient Mice Transplanted with Human CD34+ Hematopoietic Stem Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Sato, Yoshinori
× Sato, Yoshinori× 高田, 比呂志× 小林, 直樹× Nagata, Sayaka× 中潟, 直己× Ueno, Takamasa× Takiguchi, Masafumi |
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| 別言語の著者 |
佐藤, 義則
× 佐藤, 義則× 高田, 比呂志× 小林, 直樹× 永田, 紗矢香× 中潟, 直己× 上野, 貴将× 滝口, 雅文 |
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| 内容記述 | ||||||
| 内容記述 | Humanized mice, which are generated by transplanting human CD34+ hematopoietic stem cells into immunodeficient mice, are expected to be useful for the research on human immune responses. It is reported that antigen-specific T cell responses occur in immunodeficient mice transplanted with both human fetal thymus/liver tissues and CD34+ fetal cells, but it remains unclear whether antigen-specific T cell responses occur in those transplanted with only human CD34+ hematopoietic stem cells (HSCs). Here we investigated the differentiation and function of human CD8+ T cells reconstituted in NOD/SCID/Jak3-/- mice transplanted with human CD34+ HSCs (hNOK mice). Multicolor flow cytometric analysis demonstrated that human CD8+T cells generated from the CD34+ HSCs comprised only 3 subtypes, i.e., CD27highCD28+CD45RA++CCR7+, CD27+CD28++CD45R-A2CCR7+, and CD27 +CD28+CD45RA-CCR7- and had 3 phenotypes for 3 lytic molecules, i.e., perforin(Per)-granzymeA(GraA)-granzymeB(GraB)-, Per-GraA+GraB-, and PerlowGraA+GraB+. These CD8+ T cells failed to produce IFN-γ and to proliferate after stimulation with alloantigens. These results indicate that the antigen-specific T cell response cannot be elicited in mice transplanted with only human CD34+ HSCs, because the T cells fail to develop normally in such mice. | |||||
| 書誌情報 |
PLoS ONE 巻 5, 号 10, 発行年 2010-10-01 |
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| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 関連識別子 | 10.1371/journal.pone.0013109 | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 19326203 | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 1502237 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 493.87 | |||||
| 出版者 | ||||||
| 出版者 | Public Library of Science | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
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| 資源タイプ・ローカル表示コード | ||||||
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| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0013109@@@20957041 | |||||
