@article{oai:kumadai.repo.nii.ac.jp:00026316,
 author = {Takada, Miyuki and 坂本, 祐輔 and Sakamoto, Yusuke and Masamoto, Kazumori and 高田, みゆき and 坂本, 祐輔 and Masamoto, Kazumori and 正元, 和盛},
 journal = {熊本大学教育学部紀要 自然科学},
 month = {Dec},
 note = {application/pdf, 論文(Article), We developed new experimental methods for detection of sugar in sieve cells of photosynthetic products and for detection of nitrate ions in vessels by using leaf veins, in order to promote the understanding on function of plant vascular bundles, that is, to learn photosynthesis and “water pathway in plants” in elementary and lower secondary schools. We used leaf veins from petioles of several herbaceous species including annual fleabane, Erigeron annuus. Those leaf veins were easily isolated from the petioles, and used as the samples for detection of nitrate ions in them with using kits for examination of water. Nitrate ions in soil were also detected with those kits. If these experiments were introduced in “water pathway in plants” of the learning unit in science classes, then realistic understanding by these experiments on function of transportation of inorganic nutrients in plant vessels would be promoted. The sucrose content in the vein samples was compared with that in mesophyll-cell samples. It was measured with a photometer using a glucose detection reagent on the market, after conversion of sucrose into glucose by invertase. The sucrose content in the petiole veins of annual fleabane was higher than that in leaf mesophyll-cells. The detection method of sucrose was simplified for usage in elementary and lower secondary school science classes, and would promote the understanding of the conversion of starch into sugar. Those methods for detection of sucrose and nitrate ions in the veins of petioles provide new teaching materials for learning translocation of sugar in sieve tubes and translocation of inorganic nutrient in vessels},
 pages = {31--39},
 title = {児童生徒にできる葉脈を用いた転流糖、無機養分の検出実験 : 植物維管束の働き理解のために},
 volume = {60},
 year = {2011},
 yomi = {サカモト, ユウスケ and マサモト, カズモリ}
}