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Cyclosporin A Stimulation of Glucose-Induced Insulin Secretion in MIN6 Cells
http://hdl.handle.net/2298/25142
http://hdl.handle.net/2298/25142592cab73-f7d5-44dd-a594-6a939564cdbc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
22-1138.pdf (2.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2012-07-18 | |||||
| タイトル | ||||||
| タイトル | Cyclosporin A Stimulation of Glucose-Induced Insulin Secretion in MIN6 Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | thesis | |||||
| 著者 |
蛯原, 賢司
× 蛯原, 賢司 |
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| 別言語の著者 |
Ebihara, Kenji
× Ebihara, Kenji |
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| 内容記述 | ||||||
| 内容記述 | Effects of the immunosuppressant cyclosporin A (CsA), a specific inhibitor of Ca2+/calmodulin-dependent protein phosphatase (PP2B), were examined with regard to the induction of insulin secretion from MIN6 cells, a glucose-responsive cell line derived from mouse insulinoma. CsA had no effect on basal insulin secretion from MIN6 cells, but did increase glucose-, tolbutamide-, and KCl-induced insulin secretion. Treatment of the cells with CsA resulted in a dose-dependent increase in insulin secretion, which was maximal at 3 microM. CsA inhibited PP2B activity in a dose-dependent manner, and the increase in insulin secretion correlated with the decrease in PP2B activity. In 32P-labeled cells, treatment with CsA for 30 min increased phosphorylation of synapsin I-like protein by 50 +/- 5.7%. As revealed by one-dimensional phosphopeptide mapping of 32P-labeled synapsin I-like protein, treatment with CsA for 30 min increased phosphorylation of site II of synapsin I-like protein by 59 +/- 8%, which is phosphorylated by calmodulin kinase II. Messenger RNAs, which hybridize with complementary DNAs of calcineurin A and B subunits from rat brain, were detected in MIN6 cells. Western blot analysis showed a 61-kDa band, which interacts with rat brain calcineurin A antibody. Similar increases in secretagogue-induced insulin secretion with CsA were observed for HIT-T15 cells. These results suggest that CsA stimulates glucose-induced insulin secretion by inhibiting the activity of PP2B, an event that may be involved in mechanisms governing glucose-induced insulin secretion via dephosphorylation of synapsin I-like protein in MIN6 cells. | |||||
| 書誌情報 |
Endocrinology 巻 137, 号 12, p. 5255-5263, 発行年 1996-12-01 |
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| 権利 | ||||||
| 権利情報 | © 1996 The Endocrine Society | |||||
| フォーマット | ||||||
| 内容記述 | application/pdf, application/pdf | |||||
| 形態 | ||||||
| 2143880 bytes, 58267 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 日本十進分類法 | ||||||
| 主題 | 377.5 | |||||
| 出版者 | ||||||
| 出版者 | The Endocrine Society | |||||
| 資源タイプ | ||||||
| 内容記述 | 学位論文(Thesis) | |||||
| 資源タイプ・ローカル | ||||||
| 博士論文 | ||||||
| 資源タイプ・NII | ||||||
| Thesis or Dissertation | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 03 | ||||||
| URL | ||||||
| 内容記述 | http://endo.endojournals.org/content/137/12/5255.abstract | |||||
| コメント | ||||||
| 平成10年3月25日学位取得 | ||||||
| 学位番号 | ||||||
| 甲博医第1138号 | ||||||
