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Cyclosporin A Stimulation of Glucose-Induced Insulin Secretion in MIN6 Cells
http://hdl.handle.net/2298/25142
http://hdl.handle.net/2298/25142592cab73-f7d5-44dd-a594-6a939564cdbc
| 名前 / ファイル | ライセンス | アクション |
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22-1138.pdf (2.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2012-07-18 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Cyclosporin A Stimulation of Glucose-Induced Insulin Secretion in MIN6 Cells | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||||||
| 資源タイプ | thesis | |||||||||||
| 著者 |
蛯原, 賢司
× 蛯原, 賢司
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| 内容記述 | ||||||||||||
| 内容記述 | Effects of the immunosuppressant cyclosporin A (CsA), a specific inhibitor of Ca2+/calmodulin-dependent protein phosphatase (PP2B), were examined with regard to the induction of insulin secretion from MIN6 cells, a glucose-responsive cell line derived from mouse insulinoma. CsA had no effect on basal insulin secretion from MIN6 cells, but did increase glucose-, tolbutamide-, and KCl-induced insulin secretion. Treatment of the cells with CsA resulted in a dose-dependent increase in insulin secretion, which was maximal at 3 microM. CsA inhibited PP2B activity in a dose-dependent manner, and the increase in insulin secretion correlated with the decrease in PP2B activity. In 32P-labeled cells, treatment with CsA for 30 min increased phosphorylation of synapsin I-like protein by 50 +/- 5.7%. As revealed by one-dimensional phosphopeptide mapping of 32P-labeled synapsin I-like protein, treatment with CsA for 30 min increased phosphorylation of site II of synapsin I-like protein by 59 +/- 8%, which is phosphorylated by calmodulin kinase II. Messenger RNAs, which hybridize with complementary DNAs of calcineurin A and B subunits from rat brain, were detected in MIN6 cells. Western blot analysis showed a 61-kDa band, which interacts with rat brain calcineurin A antibody. Similar increases in secretagogue-induced insulin secretion with CsA were observed for HIT-T15 cells. These results suggest that CsA stimulates glucose-induced insulin secretion by inhibiting the activity of PP2B, an event that may be involved in mechanisms governing glucose-induced insulin secretion via dephosphorylation of synapsin I-like protein in MIN6 cells. | |||||||||||
| 書誌情報 |
Endocrinology 巻 137, 号 12, p. 5255-5263, 発行年 1996-12-01 |
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| 権利 | ||||||||||||
| 権利情報 | © 1996 The Endocrine Society | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| 日本十進分類法 | ||||||||||||
| 主題 | 377.5 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 熊本大学 | |||||||||||
| 言語 | ja | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士（医学）(ja) | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関名 | 熊本大学 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 1996-12-01 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第1138号 | |||||||||||
| URL | ||||||||||||
| 内容記述 | http://endo.endojournals.org/content/137/12/5255.abstract | |||||||||||
| 言語 | en | |||||||||||
| コメント | ||||||||||||
| 値 | 平成10年3月25日学位取得 | |||||||||||
| 学位番号 | ||||||||||||
| 値 | 甲博医第1138号 | |||||||||||
