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In Vivo Correction with Recombinant Adenovirus of 4-Hydroxyphenylpyruvic Acid Dioxygenase Deficiencies in Strain III Mice
http://hdl.handle.net/2298/25143
http://hdl.handle.net/2298/251431befc71a-513f-4d3c-a1bc-ea5d87b27bb2
| 名前 / ファイル | ライセンス | アクション |
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22-1146.pdf (3.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2012-07-18 | |||||||||||
| タイトル | ||||||||||||
| タイトル | In Vivo Correction with Recombinant Adenovirus of 4-Hydroxyphenylpyruvic Acid Dioxygenase Deficiencies in Strain III Mice | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||||||
| 資源タイプ | thesis | |||||||||||
| 著者 |
久保, 秀司
× 久保, 秀司
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| 内容記述 | ||||||||||||
| 内容記述 | Tyrosinemia type 3, caused by a genetic deficiency of 4-hydroxyphenylpyruvic acid dioxygenase (HPD) in tyrosine catabolism, is characterized by convulsion, ataxia, and mental retardation. The III mouse is a model of tyrosinemia type 3. HPD activity and protein are defective in the liver and its blood tyrosine levels are elevated, the range being between 1,100 and 1,656 μM. We constructed a recombinant adenoviral vector bearing the human HPD cDNA (AdexCAGhHPD), which is expressed under the control of a potent CAG promoter. III mice were injected with 1.0 × 108 to 1.0 × 109 pfu of AdexCAGhHPD through the tail vein. When 3.0 × 108–1.0 × 109 pfu were injected, blood tyrosine levels decreased within 3 hr, reached a normal range (under 300 μM), and remained at a low level for 2–6 weeks. Hepatic HPD activities also increased as early as 3 hr after the injection of 5.0 × 108 pfu, reached the levels comparable to the control mice in 3–7 days, and then decreased, and correlated well to blood tyrosine. Hepatic HPD expression was confirmed by Northern blot and immunoblot analyses. Histology revealed no difference (gross or microscopic) between the liver injected with AdexCAGhHPD and the control. No significant changes in blood tyrosine levels were noted after the second injection of 5.0 × 108 pfu of AdexCAGhHPD. Thus, the intravenous administration of the adenoviral vector bearing a foreign gene seems suitable for transient, early gene transfer into the liver. | |||||||||||
| 書誌情報 |
Human gene therapy 巻 8, 号 1, p. 65-71, 発行年 1997-01-01 |
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| 権利 | ||||||||||||
| 権利情報 | © 1997 Mary Ann Liebert, Inc. | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| 日本十進分類法 | ||||||||||||
| 主題 | 377.5 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 熊本大学 | |||||||||||
| 言語 | ja | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士（医学）(ja) | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関名 | 熊本大学 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 1997-01-01 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第1146号 | |||||||||||
| URL | ||||||||||||
| 内容記述 | http://online.liebertpub.com/doi/abs/10.1089/hum.1997.8.1-65 | |||||||||||
| 言語 | en | |||||||||||
| コメント | ||||||||||||
| 値 | 平成10年3月25日学位取得 | |||||||||||
| 学位番号 | ||||||||||||
| 値 | 甲博医第1146号 | |||||||||||
