@article{oai:kumadai.repo.nii.ac.jp:00026644,
 author = {澤, 智裕 and Ihara, Hideshi and 居原, 秀 and 笠松, 真吾 and 吉武, 淳 and 岡本, 竜哉 and 藤井, 重元 and 赤池, 孝章 and Ahmed, Khandaker Ahtesham and 澤, 智裕 and Sawa, Tomohiro and Ihara, Hideshi and Kasamatsu, Shingo and Yoshitake, Jun and Rahaman, Md. Mizanur and 岡本, 竜哉 and Okamoto, Tatsuya and 藤井, 重元 and Fujii, Shigemoto and 赤池, 孝章 and Akaike, Takaaki},
 issue = {2},
 journal = {Biochemical Journal},
 month = {Jan},
 note = {application/pdf, application/pdf, 論文(Article), 8-Nitro-cGMP (8-nitroguanosine 3′,5′-cyclic monophosphate) is a nitrated derivative of cGMP, which can function as a unique electrophilic second messenger involved in regulation of an antioxidant adaptive response in cells. In the present study, we investigated chemical and biochemical regulatory mechanisms involved in 8-nitro-cGMP formation, with particular focus on the roles of ROS (reactive oxygen species). Chemical analyses demonstrated that peroxynitrite-dependent oxidation and myeloperoxidase-dependent oxidation of nitrite in the presence of H 2O 2 were two major pathways for guanine nucleotide nitration. Among the guanine nucleotides examined, GTP was the most sensitive to peroxynitrite-mediated nitration. Immunocytochemical and tandem mass spectrometric analyses revealed that formation of 8-nitro-cGMP in rat C6 glioma cells stimulated with lipopolysaccharide plus pro-inflammatory cytokines depended on production of both superoxide and H 2O 2. Using the mitochondria-targeted chemical probe MitoSOX™ Red, we found that mitochondria-derived superoxide can act as a direct determinant of 8-nitro-cGMP formation. Furthermore, we demonstrated that Nox2 (NADPH oxidase 2)-generated H 2O 2regulated mitochondria-derived superoxide production, which suggests the importance of cross-talk between Nox2-dependent H 2O 2 production and mitochondrial superoxide production. The results of the present study suggest that 8-nitro-cGMP can serve as a unique second messenger thatmay be implicated in regulating ROS signalling in the presence of NO., http://www.biochemj.org/bj/441/bj4410719.htm},
 pages = {719--730},
 title = {Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signaling},
 volume = {441},
 year = {2012},
 yomi = {イハラ, ヒデシ and サワ, トモヒロ and オカモト, タツヤ and フジイ, シゲモト and アカイケ, タカアキ}
}