{"created":"2023-06-19T09:14:29.860370+00:00","id":26691,"links":{},"metadata":{"_buckets":{"deposit":"0430b364-293b-4410-b19b-48b5904f932e"},"_deposit":{"created_by":1,"id":"26691","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"26691"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00026691","sets":["415:416"]},"author_link":["121072","121069","121066","121062","121070","121071","121063","121073","121068","121065","121067","121064"],"item_16_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-02-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"1433","bibliographicPageStart":"1425","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"Bioorganic and Medicinal Chemistry"}]}]},"item_16_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"本山, 敬一"}],"nameIdentifiers":[{"nameIdentifier":"121070","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"林田, 佳代子"}],"nameIdentifiers":[{"nameIdentifier":"121071","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東, 大志"}],"nameIdentifiers":[{"nameIdentifier":"121072","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"有馬, 英俊"}],"nameIdentifiers":[{"nameIdentifier":"121073","nameIdentifierScheme":"WEKO"}]}]},"item_16_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_16_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_16_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Nonviral gene delivery suffers from a number of limitations including short transgene expression times and low transfection efficiency. In this study, we examined whether polypseudorotaxanes (PPRXs) of polyethylene glycol (PEG, molecular weight: 2,000)-grafted α-cyclodextrin (α-CyD)/ polyamidoamine dendrimer conjugate (PEG-α-CDE) with CyDs have the potential for the novel sustained release systems for plasmid DNA (pDNA). The PEG-α-CDE/pDNA complex formed PPRXs with α-CyD and γ-CyD solutions, but not with β-CyD solution. In the PEG-α-CDE/CyDs PPRX systems, 20.6 mol of α-CyD and 11.8 mol of γ-CyD were involved in the PPRXs formation with one PEG chain by α-CyD and γ-CyD, respectively, consistent with in the PEG-dendrimer/CyDs systems. PEG-α-CDE/pDNA/α-CyD PPRX and PEG-α-CDE/pDNA/γ-CyD PPRX formed hexagonal and tetragonal columnar channels in the crystalline phase, respectively. In addition, the CyDs PPRX provided the sustained release of pDNA from PEG-α-CDE complex with pDNA at least 72 h in vitro. The release of pDNA from CyDs PPRX retarded as the volume of dissolution medium decreased. Furthermore, the PEG-α-CDE/γ-CyD PPRX system showed sustained transfection efficiency after intramuscular injection to mice at least for 14 days. These results suggest that the PEG-α-CDE/CyD PPRX systems are useful for novel sustained DNA release systems.","subitem_description_type":"Other"}]},"item_16_description_77":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_description":"http://www.sciencedirect.com/science/article/pii/S0968089612000028","subitem_description_type":"Other"}]},"item_16_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Limited"}]},"item_16_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"22277591","subitem_relation_type_select":"PMID"}}]},"item_16_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bmc.2011.12.060","subitem_relation_type_select":"DOI"}}]},"item_16_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 Elsevier Limited"}]},"item_16_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09680896","subitem_source_identifier_type":"ISSN"}]},"item_16_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491.7","subitem_subject_scheme":"NDC"}]},"item_16_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"640544 bytes"}]},"item_16_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"雑誌掲載論文"}]},"item_16_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Journal Article"}]},"item_16_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_16_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"01"}]},"item_16_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Motoyama, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"121062","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayashida, Kayoko"}],"nameIdentifiers":[{"nameIdentifier":"121063","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higashi, Taishi"}],"nameIdentifiers":[{"nameIdentifier":"121064","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arima, Hidetoshi"}],"nameIdentifiers":[{"nameIdentifier":"121065","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"Bioorganic and Medicinal Chemistry2012.VOL20-4.pdf","filesize":[{"value":"640.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bioorganic and Medicinal Chemistry2012.VOL20-4.pdf","url":"https://kumadai.repo.nii.ac.jp/record/26691/files/Bioorganic and Medicinal Chemistry2012.VOL20-4.pdf"},"version_id":"a1c92be3-8bb7-4d2e-88e8-2185d627f24c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cyclodextrin","subitem_subject_scheme":"Other"},{"subitem_subject":"polypseudorotaxane","subitem_subject_scheme":"Other"},{"subitem_subject":"dendrimer","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"sustained release","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Polypseudorotaxanes of Pegylated α-Cyclodextrin/Polyamidoamine Dendrimer Conjugate with Cyclodextrins as a Sustained Release System for DNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Polypseudorotaxanes of Pegylated α-Cyclodextrin/Polyamidoamine Dendrimer Conjugate with Cyclodextrins as a Sustained Release System for DNA"}]},"item_type_id":"16","owner":"1","path":["416"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-12-11"},"publish_date":"2012-12-11","publish_status":"0","recid":"26691","relation_version_is_last":true,"title":["Polypseudorotaxanes of Pegylated α-Cyclodextrin/Polyamidoamine Dendrimer Conjugate with Cyclodextrins as a Sustained Release System for DNA"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T17:47:45.822722+00:00"}