{"created":"2023-06-19T09:14:33.219689+00:00","id":26746,"links":{},"metadata":{"_buckets":{"deposit":"a4f3b41c-4922-4a01-9579-4db49f115938"},"_deposit":{"created_by":1,"id":"26746","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"26746"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00026746","sets":["415:416"]},"author_link":["121520","121522","121517","121516","121526","121513","121512","121525","121510","121511","121521","121515","121514","121519","121509","121518","121523","121524"],"item_16_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-04-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"247","bibliographicPageStart":"239","bibliographicVolumeNumber":"426","bibliographic_titles":[{"bibliographic_title":"International Journal of Pharmaceutics"}]}]},"item_16_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安野, 貴幸"}],"nameIdentifiers":[{"nameIdentifier":"121521","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東, 大志"}],"nameIdentifiers":[{"nameIdentifier":"121522","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"本山, 敬一"}],"nameIdentifiers":[{"nameIdentifier":"121523","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平山, 文俊"}],"nameIdentifiers":[{"nameIdentifier":"121524","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上釜, 兼人"}],"nameIdentifiers":[{"nameIdentifier":"121525","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"有馬, 英俊"}],"nameIdentifiers":[{"nameIdentifier":"121526","nameIdentifierScheme":"WEKO"}]}]},"item_16_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"},{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_16_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_16_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"We previously reported that glucuronylglucosyl-β-cyclodextrin (GUG-β-CyD) conjugate with polyamidoamine starburst dendrimer (GUG-β-CDE conjugate) with the average degree of substitution (DS) of cyclodextrin (CyD) of 1.8 (GUG-β-CDE conjugate (DS 1.8)), showed remarkably higher gene transfer activity than α-cyclodextrin/dendrimer conjugate (α-CDE conjugate (DS 1.2)) and β-cyclodextrin/dendrimer conjugate (β-CDE conjugate (DS 1.3)) in vitro and in vivo. In this study, to clarify the enhancing mechanism for high gene transfer activity of GUG-β-CDE conjugate (DS 1.8), we investigated the physicochemical properties, cellular uptake, endosomal escape and nuclear translocation of the plasmid DNA (pDNA) complexes as well as pDNA release from the complexes. The particle size, ς-potential and cellular uptake of GUG-β-CDE conjugate (DS 1.8)/pDNA complex were mostly comparable to those of α-CDE conjugate (DS 1.2) and β-CDE conjugate (DS 1.3). Meanwhile, GUG-β-CDE conjugate (DS 1.8)/pDNA complex was likely to have high endosomal escaping ability and nuclear localization ability in A549 and RAW264.7 cells. In addition, the pDNA condensation and decondensation abilities of GUG-β-CDE conjugate (DS 1.8) were lower and higher than that of α-CDE conjugate (DS 1.2) or β-CDE conjugate (DS 1.3), respectively. These results suggest that high gene transfer activity of GUG-β-CDE conjugate (DS 1.8) could be, at least in part, attributed to high endosomal escaping ability, nuclear localization ability and suitable pDNA release of its complex.","subitem_description_type":"Other"}]},"item_16_description_77":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_description":"http://www.sciencedirect.com/science/article/pii/S0378517312000932","subitem_description_type":"Other"}]},"item_16_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_16_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"22306427","subitem_relation_type_select":"PMID"}}]},"item_16_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ijpharm.2012.01.039","subitem_relation_type_select":"DOI"}}]},"item_16_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 Elsevier"}]},"item_16_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03785173","subitem_source_identifier_type":"ISSN"}]},"item_16_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"499","subitem_subject_scheme":"NDC"}]},"item_16_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"352502 bytes"},{"subitem_text_value":"324824 bytes"}]},"item_16_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"雑誌掲載論文"}]},"item_16_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Journal 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Kaneto"}],"nameIdentifiers":[{"nameIdentifier":"121513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arima, Hidetoshi"}],"nameIdentifiers":[{"nameIdentifier":"121514","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"Fig-IJP-GUG-R1-submit.pdf","filesize":[{"value":"324.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Fig-IJP-GUG-R1-submit.pdf","url":"https://kumadai.repo.nii.ac.jp/record/26746/files/Fig-IJP-GUG-R1-submit.pdf"},"version_id":"d9431139-421b-4139-81d5-0d492a334120"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"IJP-Anno-arima-Kumamoto University-Archive-2.pdf","filesize":[{"value":"352.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJP-Anno-arima-Kumamoto University-Archive-2.pdf","url":"https://kumadai.repo.nii.ac.jp/record/26746/files/IJP-Anno-arima-Kumamoto University-Archive-2.pdf"},"version_id":"138ff594-cc96-4f8f-a02e-746ad14de2b0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"PAMAM starburst dendrimer","subitem_subject_scheme":"Other"},{"subitem_subject":"glucuronylglucosyl-β-cyclodextrin","subitem_subject_scheme":"Other"},{"subitem_subject":"conjugate","subitem_subject_scheme":"Other"},{"subitem_subject":"cyclodextrins","subitem_subject_scheme":"Other"},{"subitem_subject":"non-viral vector","subitem_subject_scheme":"Other"},{"subitem_subject":"enhancing mechanism","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Possible Enhancing Mechanisms for Gene Transfer Activity of Glucuronylglucosyl-β-cyclodextrin/dendrimer Conjugate","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Possible Enhancing Mechanisms for Gene Transfer Activity of Glucuronylglucosyl-β-cyclodextrin/dendrimer Conjugate"}]},"item_type_id":"16","owner":"1","path":["416"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-01-17"},"publish_date":"2013-01-17","publish_status":"0","recid":"26746","relation_version_is_last":true,"title":["Possible Enhancing Mechanisms for Gene Transfer Activity of Glucuronylglucosyl-β-cyclodextrin/dendrimer Conjugate"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T17:47:04.225853+00:00"}