{"created":"2023-06-19T09:15:16.647544+00:00","id":27618,"links":{},"metadata":{"_buckets":{"deposit":"9e20f8c0-4ab5-42c9-a1ad-c4503093ecf1"},"_deposit":{"created_by":1,"id":"27618","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"27618"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00027618","sets":["413:414","585:612:1260"]},"author_link":["157754","157658","157752","157659","157753"],"item_15_alternative_title_22":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Establishment of an inducible M12-Tet-Off pTRE2pur FLAG mTRAF3 cell"}]},"item_15_alternative_title_23":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"M12 Tet Off pTRE2pur FLAG mTRAF3 サイボウカブ ノ ジュリツ"}]},"item_15_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-03-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"38","bibliographicPageStart":"27","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"熊本大学医学部保健学科紀要"}]}]},"item_15_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"田邊, 香野","creatorNameLang":"ja"},{"creatorName":"タナベ, カノ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanabe, Kano","creatorNameLang":"en"}],"familyNames":[{"familyName":"田邊","familyNameLang":"ja"},{"familyName":"タナベ","familyNameLang":"ja-Kana"},{"familyName":"Tanabe","familyNameLang":"en"}],"givenNames":[{"givenName":"香野","givenNameLang":"ja"},{"givenName":"カノ","givenNameLang":"ja-Kana"},{"givenName":"Kano","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"157658","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"坂本, 瞳","creatorNameLang":"ja"},{"creatorName":"サカモト, ヒトミ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakamoto, Hitomi","creatorNameLang":"en"}],"familyNames":[{"familyName":"坂本","familyNameLang":"ja"},{"familyName":"サカモト","familyNameLang":"ja-Kana"},{"familyName":"Sakamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"瞳","givenNameLang":"ja"},{"givenName":"ヒトミ","givenNameLang":"ja-Kana"},{"givenName":"Hitomi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"157753","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"乾, 誠治","creatorNameLang":"ja"},{"creatorName":"イヌイ, セイジ","creatorNameLang":"ja-Kana"},{"creatorName":"Inui, Seiji","creatorNameLang":"en"}],"familyNames":[{"familyName":"乾","familyNameLang":"ja"},{"familyName":"イヌイ","familyNameLang":"ja-Kana"},{"familyName":"Inui","familyNameLang":"en"}],"givenNames":[{"givenName":"誠治","givenNameLang":"ja"},{"givenName":"セイジ","givenNameLang":"ja-Kana"},{"givenName":"Seiji","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"157659","nameIdentifierScheme":"WEKO"}]}]},"item_15_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_15_description_46":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_15_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"IL-4 induces IgE class switching in B cells together with CD40 stimulation. Studies on the signal transduction mechanism downstream of IL-4 receptor have revealed that NF-κB as well as Jak-STAT pathway plays an important role. We showed the possibility that alternative pathway of NF-κB was activated after IL-4 stimulation of murine B cell line M12. Alternative pathway of NF-κB is strictly regulated by constant degradation of NIK, an essential molecule for NF-κB activation. TRAF3 is the key molecule to keep the alternative pathway in an inactive state. TRAF3 is also regulated by the constant degradation by ubiquitin-proteasome system. To elucidate the mechanism of the TRAF3 degradation, we prepared Tet-Off gene expression system in M12 to inducibly express TRAF3 which was toxic when overexpressed in cells. Firstly, pTet-Off vector was introduced into M12 cells by electroporation and G418 resistant clones were selected which harbored pTet-Off. Clones were further selected to show high responsiveness to Doxycyclin withdrawal using transient expression of luciferase vector. One of the clones, C'' , was transfected with pTRE2pur-mTRAF3 vector to obtain FLAG-tagged mTRAF3 expressing cells upon induction. One clone, C'' -1' , was successfully established which expressed mTRAF3 in an inducible manner.","subitem_description_type":"Other"}]},"item_15_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"熊本大学"}]},"item_15_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18807151","subitem_source_identifier_type":"ISSN"}]},"item_15_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12010572","subitem_source_identifier_type":"NCID"}]},"item_15_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"463","subitem_subject_scheme":"NDC"}]},"item_15_text_18":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_text_value":"1588446 bytes"}]},"item_15_text_47":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_15_text_48":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_15_text_49":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_15_text_50":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"02"}]},"item_15_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田邊, 香野"}],"nameIdentifiers":[{"nameIdentifier":"157752","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"坂本, 瞳","creatorNameLang":"ja"},{"creatorName":"サカモト, ヒトミ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakamoto, Hitomi","creatorNameLang":"en"}],"familyNames":[{"familyName":"坂本","familyNameLang":"ja"},{"familyName":"サカモト","familyNameLang":"ja-Kana"},{"familyName":"Sakamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"瞳","givenNameLang":"ja"},{"givenName":"ヒトミ","givenNameLang":"ja-Kana"},{"givenName":"Hitomi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"157753","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"乾, 誠治"}],"nameIdentifiers":[{"nameIdentifier":"157754","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"MH0009_027-038.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MH0009_027-038.pdf","url":"https://kumadai.repo.nii.ac.jp/record/27618/files/MH0009_027-038.pdf"},"version_id":"33a9144d-3fb9-4828-ac8a-3697e5b52827"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Tet-Off system","subitem_subject_scheme":"Other"},{"subitem_subject":"M12 cell","subitem_subject_scheme":"Other"},{"subitem_subject":"TRAF3","subitem_subject_scheme":"Other"},{"subitem_subject":"NF-kB","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立"}]},"item_type_id":"15","owner":"1","path":["414","1260"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-04-18"},"publish_date":"2013-04-18","publish_status":"0","recid":"27618","relation_version_is_last":true,"title":["M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-09-19T02:22:13.464211+00:00"}