@article{oai:kumadai.repo.nii.ac.jp:00027973,
 author = {近藤, 龍也 and 佐々木, 一成 and Sasaki, Kazunari and 松山, 利奈 and Morino-Koga, Saori and 古賀, 沙緒里 and 森野 古賀, 沙緒里 and 足立, 博紀 and 河島, 淳司 and 本島, 寛之 and 古川, 昇 and 甲斐, 広文 and 荒木, 栄一 and Araki, Eiichi and Kondo, Tatsuya and 佐々木, 一成 and Sasaki, Kazunari and Matsuyama, Rina and Morino-Koga, Saori and 古賀, 沙緒里 and 森野 古賀, 沙緒里 and Adachi, Hironori and Suico, Mary A and Kawashima, Junji and Motoshima, Hiroyuki and Furukawa, Noboru and Kai, Hirofumi and 荒木, 栄一 and Araki, Eiichi},
 issue = {4},
 journal = {Diabetes},
 month = {Apr},
 note = {application/pdf, application/pdf, application/pdf, 論文(Article), Induction of heat shock protein (HSP) 72 improves metabolic profiles in diabetic model mice.
However, its impact on pancreatic β-cells is not known. The present study investigated whether
HSP72 induction can reduce β-cell stress signaling and apoptosis, and preserve β-cell mass.
MIN6 cells and db/db mice were sham-treated or treated with heat shock (HS) + mild electrical
stimulation (MES) to induce HSP72. Several cellular markers, metabolic parameters and β-cell
mass were evaluated.
HS+MES treatment or HSP72 overexpression increased the HSP72 protein levels and decreased
TNF-β-induced JNK phosphorylation, ER stress and pro-apoptotic signal in MIN6 cells. In db/db
mice, HS+MES treatment for 12 weeks significantly improved the insulin sensitivity and glucose
homeostasis. Upon glucose challenge, a significant increase in insulin secretion was observed in
vivo. Compared with sham treatment, the HSP72, insulin, PDX-1, GLUT2 and IRS-2 levels were
upregulated in the pancreatic islets of HS+MES-treated mice, whereas JNK phosphorylation,
nuclear translocation of FOXO1 and NF-βB p65 were reduced. Apoptotic signals, ER stress and
oxidative stress markers were attenuated.
Thus, HSP72 induction by HS+MES treatment protects β-cells from apoptosis by attenuating
JNK activation and cell stresses. HS+MES combination therapy may preserve pancreatic β-cell
volume to ameliorate glucose homeostasis in diabetes., http://diabetes.diabetesjournals.org/content/61/4/838.abstract},
 pages = {838--847},
 title = {Hyperthermia with Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis},
 volume = {61},
 year = {2012},
 yomi = {ササキ, カズナリ and コガ, サオリ and アラキ, エイイチ and ササキ, カズナリ and コガ, サオリ and アラキ, エイイチ}
}