{"created":"2023-06-19T09:15:35.245706+00:00","id":27973,"links":{},"metadata":{"_buckets":{"deposit":"db65ab7a-c6f4-4757-8995-a9f3866f7af7"},"_deposit":{"created_by":127,"id":"27973","owners":[127],"pid":{"revision_id":0,"type":"depid","value":"27973"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00027973","sets":["413:443"]},"author_link":[],"item_16_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"847","bibliographicPageStart":"838","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"Diabetes","bibliographic_titleLang":"en"}]}]},"item_16_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Induction of heat shock protein (HSP) 72 improves metabolic profiles in diabetic model mice. However, its impact on pancreatic β-cells is not known. The present study investigated whether HSP72 induction can reduce β-cell stress signaling and apoptosis, and preserve β-cell mass. MIN6 cells and db/db mice were sham-treated or treated with heat shock (HS) + mild electrical stimulation (MES) to induce HSP72. Several cellular markers, metabolic parameters and β-cell mass were evaluated. HS+MES treatment or HSP72 overexpression increased the HSP72 protein levels and decreased TNF-β-induced JNK phosphorylation, ER stress and pro-apoptotic signal in MIN6 cells. In db/db mice, HS+MES treatment for 12 weeks significantly improved the insulin sensitivity and glucose homeostasis. Upon glucose challenge, a significant increase in insulin secretion was observed in vivo. Compared with sham treatment, the HSP72, insulin, PDX-1, GLUT2 and IRS-2 levels were upregulated in the pancreatic islets of HS+MES-treated mice, whereas JNK phosphorylation, nuclear translocation of FOXO1 and NF-βB p65 were reduced. Apoptotic signals, ER stress and oxidative stress markers were attenuated. Thus, HSP72 induction by HS+MES treatment protects β-cells from apoptosis by attenuating JNK activation and cell stresses. HS+MES combination therapy may preserve pancreatic β-cell volume to ameliorate glucose homeostasis in diabetes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_16_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Diabetes Association","subitem_publisher_language":"en"}]},"item_16_relation_61":{"attribute_name":"関連","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"22362176","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2337/db11-1098","subitem_relation_type_select":"DOI"}}]},"item_16_source_id_7":{"attribute_name":"item_16_source_id_7","attribute_value_mlt":[{"subitem_source_identifier":"0012-1797","subitem_source_identifier_type":"PISSN"}]},"item_16_source_id_9":{"attribute_name":"item_16_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"AA00628057","subitem_source_identifier_type":"NCID"}]},"item_16_subject_20":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"493","subitem_subject_scheme":"NDC"}]},"item_16_version_type_19":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kondo, Tatsuya","creatorNameLang":"en"},{"creatorName":"近藤, 龍也","creatorNameLang":"ja"},{"creatorName":"コンドウ, タツヤ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Sasaki, Kazunari","creatorNameLang":"en"},{"creatorName":"佐々木, 一成","creatorNameLang":"ja"},{"creatorName":"ササキ, カズナリ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Matsuyama, Rina","creatorNameLang":"en"},{"creatorName":"松山, 利奈","creatorNameLang":"ja"},{"creatorName":"マツヤマ, リナ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Morino-Koga, Saori","creatorNameLang":"en"},{"creatorName":"森野 古賀, 沙緒里","creatorNameLang":"ja"},{"creatorName":"モリノ コガ, サオリ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Adachi, Hironori","creatorNameLang":"en"},{"creatorName":"足立, 博紀","creatorNameLang":"ja"},{"creatorName":"アダチ, ヒロノリ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Suico, Mary A","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawashima, Junji","creatorNameLang":"en"},{"creatorName":"河島, 淳司","creatorNameLang":"ja"},{"creatorName":"カワシマ, ジュンジ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Motoshima, Hiroyuki","creatorNameLang":"en"},{"creatorName":"本島, 寛之","creatorNameLang":"ja"},{"creatorName":"モトシマ, ヒロユキ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Furukawa, Noboru","creatorNameLang":"en"},{"creatorName":"古川, 昇","creatorNameLang":"ja"},{"creatorName":"フルカワ, ノボル","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Kai, Hirofumi","creatorNameLang":"en"},{"creatorName":"甲斐, 広文","creatorNameLang":"ja"},{"creatorName":"カイ, ヒロフミ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Araki, Eiichi","creatorNameLang":"en"},{"creatorName":"荒木, 栄一","creatorNameLang":"ja"},{"creatorName":"アラキ, エイイチ","creatorNameLang":"ja-Kana"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-03-03"}],"displaytype":"detail","filename":"Figures-1.pdf","filesize":[{"value":"745.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Figures-1.pdf","url":"https://kumadai.repo.nii.ac.jp/record/27973/files/Figures-1.pdf"},"version_id":"455cf8db-99b9-4a6e-8620-394a51369ae4"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-03-03"}],"displaytype":"detail","filename":"Supplemental data for Diabetes _Kondo et al_-2.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Supplemental data for Diabetes _Kondo et al_-2.pdf","url":"https://kumadai.repo.nii.ac.jp/record/27973/files/Supplemental data for Diabetes _Kondo et al_-2.pdf"},"version_id":"d7a154ae-a59b-4fc6-86fb-3a8321361d30"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-03-03"}],"displaytype":"detail","filename":"MET-beta for Diabetes _Kondo et al_ -clean copy-1.pdf","filesize":[{"value":"149.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MET-beta for Diabetes _Kondo et al_ -clean copy-1.pdf","url":"https://kumadai.repo.nii.ac.jp/record/27973/files/MET-beta for Diabetes _Kondo et al_ -clean copy-1.pdf"},"version_id":"1ee1224e-acee-4290-a5d0-c9d38863618b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hyperthermia with Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hyperthermia with Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis","subitem_title_language":"en"}]},"item_type_id":"16","owner":"127","path":["443"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-01-29"},"publish_date":"2014-01-29","publish_status":"0","recid":"27973","relation_version_is_last":true,"title":["Hyperthermia with Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis"],"weko_creator_id":"127","weko_shared_id":-1},"updated":"2025-05-01T05:11:48.167978+00:00"}