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The impact of Ca2+/calmodulin-dependent protein kinase II on insulin gene expression in MIN6 cells
http://hdl.handle.net/2298/29505
http://hdl.handle.net/2298/295058ac3a6ed-d72b-4341-8671-9018a78dd87a
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2014-03-06 | |||||
| タイトル | ||||||
| タイトル | The impact of Ca2+/calmodulin-dependent protein kinase II on insulin gene expression in MIN6 cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | CaMKIIδ2, CREB, CREB binding protein, CREB Ser142, Phosphorylation, Insulin gene promoter | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Suefuji, Mihoshi
× Suefuji, Mihoshi× Furukawa, Noboru× 松本, 和也× Oiso, Hiroshi× Shimoda, Seiya× Yoshinaga, Tomoaki× Matsuyama, Rina× Miyagawa, Katsutoshi× Kondo, Tatsuya× Kawashima, Junji× Tsuruzoe, Kaku× 荒木, 栄一 |
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| 別言語の著者 |
末藤, 美星
× 末藤, 美星× 古川, 昇× 松本, 和也× 大礒, 洋× 下田, 誠也× 吉永, 智昭× 松山, 利奈× 宮川, 克俊× 近藤, 龍也× 河島, 淳司× 水流添, 覚× 荒木, 栄一 |
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| 内容記述 | ||||||
| 内容記述 | Ca2+/calmodulin-dependent protein kinase II (CaMKII) is expressed in insulin-secreting β cells. However, the effects of CaMKII on insulin synthesis are unknown. Although Ser133 phosphorylation of cyclic AMP-responsive element-binding (CREB) typically increases CREB transcriptional activity, CaMKII phosphorylates CREB at Ser142 and at Ser133 to exert a dominant inhibitory effect. Our objective was to characterize the role of CaMKII in insulin gene expression. In MIN6 cells, insulin gene promoter activity was significantly down-regulated by wild-type (WT) CaMKIIδ2, but was significantly upregulated after small interfering RNA (siRNA) knockdown of CaMKIIδ expression. These results were independent of glucose concentrations and membrane depolarization. Insulin mRNA levels were also decreased by WT CaMKIIδ2 and increased by CaMKIIδ siRNA. Downregulation of insulin gene promoter activity by WT CaMKIIδ2 was partly mediated via cyclic AMP-responsive element 2. WT CaMKIIδ2 significantly increased CREB phosphorylation at Ser142 and significantly decreased binding to CREB binding protein (CBP), whereas kinase dead CaMKIIδ2 did not. Our results indicate that CaMKIIδ2 downregulates insulin gene expression by Ser142 phosphorylation of CREB and reducing binding of CREB to CBP. |
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| 書誌情報 |
Biochemical and Biophysical Research Communications 巻 421, 号 4, p. 801-807, 発行年 2012-05-18 |
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| ISSN | ||||||
| 収録物識別子 | 0006291X | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 22554507 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1016/j.bbrc.2012.04.091 | |||||
| 権利 | ||||||
| 権利情報 | @Elsevier | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
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| 出版タイプ | AM | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 493 | |||||
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| 出版者 | Elsevier | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
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| 雑誌掲載論文 | ||||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.sciencedirect.com/science/article/pii/S0006291X12007735 | |||||
