WEKO3
アイテム
Translational Repression of a Splice Variant of Cynomolgus Macaque CXCL1L by Its C-terminal Sequence
http://hdl.handle.net/2298/39222
http://hdl.handle.net/2298/392221d10cf14-8974-4b33-817b-eee73d360d3a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Nomiyama_et_al(JICR).pdf (2.5 MB)
|
|
| Item type | プレプリント / Preprint(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-03-07 | |||||
| タイトル | ||||||
| タイトル | Translational Repression of a Splice Variant of Cynomolgus Macaque CXCL1L by Its C-terminal Sequence | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | chemokine, CXCL1L, translational regulation, gene duplication, evolution, cynomolgus macaque, Old World monkeys, ケモカイン, 翻訳制御, 遺伝子重複, 進化, カニクイザル, 旧世界ザル | |||||
| 資源タイプ | ||||||
| 資源タイプ | other | |||||
| 著者 |
Nomiyama, Hisayuki
× Nomiyama, Hisayuki× Osada, Naoki× Takahashi, Ichiro× Terao, Keiji× Yamagata, Kazuya× Yoshie, Osamu |
|||||
| 別言語の著者 |
野見山, 尚之
× 野見山, 尚之× 長田, 直樹× 高橋, 一郎× 寺尾, 恵治× 山縣, 和也× 義江, 修 |
|||||
| 内容記述 | ||||||
| 内容記述 | We previously isolated a cDNA clone from cynomolgus macaque encoding a novel CXC chemokine that we termed CXCL1L from its close similarity to CXCL1. However, the cDNA consisted of three exons instead of four exons typically seen in other CXC chemokines. Here we isolated a cDNA encoding the full length variant of CXCL1L that we term CXCL1Lβ. CXCL1Lβ is 50 amino acids longer than the original CXCL1L, which we now term CXCL1Lα. The CXCL1Lβ mRNA is much more abundantly expressed in the cynomolgus macaque tissues than CXCL1Lα mRNA. However, CXCL1Lβ protein was poorly produced by transfected cells compared with that of CXCL1Lα. When the coding region of the 4th exon was fused to the C-terminus of CXCL1 or even to a non-secretory protein firefly luciferase, the fused proteins were also barely produced although the mRNAs were abundantly expressed. The polysome profiling analysis suggested that the inhibition was mainly at the translational level. Furthermore, we demonstrated the C-terminal five amino acids of CXCL1Lβ were critical for the translational repression. The present study thus reveals a unique translational regulation controlling the production of a splicing variant of CXCL1L. Since the CXCL1L gene is functional only in the Old World monkeys, we also discuss possible reasons for the conservation of the active CXCL1L gene in these monkeys during the primate evolution. | |||||
| 書誌情報 |
Journal of Interferon & Cytokine Research 巻 37, 号 3, p. 129-138, 発行日 2017-03-17 |
|||||
| ISSN | ||||||
| 収録物識別子 | 10799907 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | https://doi.org/10.1089/jir.2016.0085 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 2488149 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | AO | |||||
| 出版タイプResource | http://purl.org/coar/version/c_b1a7d7d4d402bcce | |||||
| 出版者 | ||||||
| 出版者 | Mary Ann Liebert | |||||
| 資源タイプ・ローカル | ||||||
| プレプリント | ||||||
| 資源タイプ・NII | ||||||
| Preprint | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 01 | ||||||
| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | https://www.liebertpub.com/doi/10.1089/jir.2016.0085 | |||||
