@article{oai:kumadai.repo.nii.ac.jp:00029745,
 author = {新森, 加納子 and 中村, 富美子 and Nakamura, Fumiko and 小泉, 大海 and 新森, 大佑 and Niimori-Kita, Kanako and 中村, 富美子 and Nakamura, Fumiko and Koizumi, Daikai and Niimori, Daisuke},
 issue = {1},
 journal = {Journal of Bioanalysis & Biomedicine},
 month = {May},
 note = {application/pdf, 論文(Article), Smoking is a risk factor of lung diseases including chronic obstructive pulmonary disease (COPD) and lung
cancer. However, the molecular mechanisms inducing these diseases remain to be completely uncovered. In
order to elucidate them, it is necessary to identify the signaling pathway activated by tobacco smoking exposure.
Especially, it is important to identify nuclear phosphoproteins induced by tobacco smoking exposure because the
signaling pathways are modified by phosphoproteins. This time, to identify nuclear phosphoproteins as novel smoking
markers, nuclear phosphoproteimics of mouse lung tissue following tobacco smoking exposure was examined.
Tobacco smoking exposure against mice was examined using the nose-only, flow-past inhalation exposure chamber
system for one month. Phosphopeptides eluted from nuclear proteins of the tobacco exposured mice lungs were
identified by mass spectrometry. The result showed that 77 phosphoproteins were totally identified. Among them, the
semiquantitative analysis using ProteoIQ proteomic software revealed that five phosphoproteins showed the different
expression patterns between control and tobacco exposure groups. Furthermore, the classification by biological
functions of the identified proteins revealed that these proteins were related to inflammation, regeneration, repair,
proliferation, differentiation, morphological change and nicotine or stress response. Finally, we founded advanced
glycosylation end product-specific receptor (RAGE) and serine/threonine-protein kinase SNF1-like kinase 2 (SIK2)
as novel smoking markers.},
 pages = {9--16},
 title = {Nuclear Phosphoproteomics Features the Novel Smoking Markers in Mouse Lung Tissue Following Subacute Phase Exposure to Tobacco Smoke},
 volume = {8},
 year = {2016},
 yomi = {ナカムラ, フミコ and ナカムラ, フミコ}
}