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Inactivation of Enterobacter Aerogenes in Carboxymethyl Cellulose Solution using Intense Pulsed Electric Fields (iPEF) Combined with Moderate Thermal Treatment
http://hdl.handle.net/2298/36342
http://hdl.handle.net/2298/36342d056c499-e0c5-4a7f-b705-52b4b0f6cf9c
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-03-08 | |||||
タイトル | ||||||
タイトル | Inactivation of Enterobacter Aerogenes in Carboxymethyl Cellulose Solution using Intense Pulsed Electric Fields (iPEF) Combined with Moderate Thermal Treatment | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | liquid sterilization, low temperature, pulsed electric field, thermal energy, Blumlein generator | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
Kajiwara, Taiga
× Kajiwara, Taiga× Oide, Tatsuya× 馬場, 一磨× Ohnishi, Nobuaki× 勝木, 淳× Akiyama, Hidenori× Sasahara, Ryo× Inoue, Kazuyuki |
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別言語の著者 |
梶原, 大河
× 梶原, 大河× 大井手, 達哉× 馬場, 一磨× 大西, 伸明× 勝木, 淳× 秋山, 秀典 |
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内容記述 | ||||||
内容記述 | This paper describes low-temperature sterilization of Enterobacter aerogenes in carboxymethyl cellulose solution using intense pulsed electric fields (iPEF) combined with moderate thermal energy. The bacterial suspension was exposed to moderate temperatures of up to 55ºC for 2 minutes after tens of 530 ns-long, 50 kV/cm pulses in a single-pass continuous flow system. Suspension temperatures at the entrance and exit of the iPEF exposure chamber were maintained at 40ºC by means of an electrode cooling system. The iPEF combined with subsequent thermal energy of 55ºC reduced bacterial population by 6.6 Log10 cycles or more, compared with a reduction of only 2.1 Log10 cycles by iPEF without heat treatment. Sterilization effects increased with increasing thermal treatment temperature and pulse number. Results obtained after culturing the iPEF-exposed bacteria in NaCl rich agar, which hinders the reorganization of the damaged membrane, implies that even bacteria surviving the iPEF exposure are damaged to some extent though may later recover. Bacteria were made vulnerable to subsequent thermal treatment by iPEF-induced membrane damage. This indicates that moderate thermal stress after iPEF exposure increases sterilization effects. |
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書誌情報 |
IEEE Transactions on Dielectrics and Electrical Insulation 巻 22, 号 4, p. 1849-1855, 発行年 2015-08-01 |
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ISSN | ||||||
収録物識別子 | 10709878 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
関連識別子 | 10.1109/TDEI.2015.005041 | |||||
権利 | ||||||
権利情報 | © Copyright 2017 IEEE | |||||
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内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
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1688543 bytes | ||||||
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出版タイプ | AM | |||||
出版者 | ||||||
出版者 | Institute of Electrical and Electronics Engineers | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | 論文(Article) | |||||
資源タイプ・ローカル | ||||||
雑誌掲載論文 | ||||||
資源タイプ・NII | ||||||
Journal Article | ||||||
資源タイプ・DCMI | ||||||
text | ||||||
資源タイプ・ローカル表示コード | ||||||
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URL | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://ieeexplore.ieee.org/document/7179140/ |