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Dynamic behavior of DNA topoisomerase IIβ in response to DNA double-strand breaks
http://hdl.handle.net/2298/40061
http://hdl.handle.net/2298/40061da514f89-512b-4c1b-907f-6c765510875a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
s41598-018-28690-6.pdf (6.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-08-06 | |||||
| タイトル | ||||||
| タイトル | Dynamic behavior of DNA topoisomerase IIβ in response to DNA double-strand breaks | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Morotomi-Yano, Keiko
× Morotomi-Yano, Keiko× 斎藤, 慎太× Adachi, Noritaka× Yano, Ken-ichi |
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| 別言語の著者 |
諸冨（矢野）, 桂子
× 諸冨（矢野）, 桂子× 斎藤, 慎太× 足立, 典隆× 矢野, 憲一 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | DNA topoisomerase II (Topo II) is crucial for resolving topological problems of DNA and plays important roles in various cellular processes, such as replication, transcription, and chromosome segregation. Although DNA topology problems may also occur during DNA repair, the possible involvement of Topo II in this process remains to be fully investigated. Here, we show the dynamic behavior of human Topo IIβ in response to DNA double-strand breaks (DSBs), which is the most harmful form of DNA damage. Live cell imaging coupled with site-directed DSB induction by laser microirradiation demonstrated rapid recruitment of EGFP-tagged Topo IIβ to the DSB site. Detergent extraction followed by immunofluorescence showed the tight association of endogenous Topo IIβ with DSB sites. Photobleaching analysis revealed that Topo IIβ is highly mobile in the nucleus. The Topo II catalytic inhibitors ICRF-187 and ICRF-193 reduced the Topo IIβ mobility and thereby prevented Topo IIβ recruitment to DSBs. Furthermore, Topo IIβ knockout cells exhibited increased sensitivity to bleomycin and decreased DSB repair mediated by homologous recombination (HR), implicating the role of Topo IIβ in HR-mediated DSB repair. Taken together, these results highlight a novel aspect of Topo IIβ functions in the cellular response to DSBs. |
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| 書誌情報 |
Scientific Reports 巻 8, p. 10344, 発行年 2018-07-09 |
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| ISSN | ||||||
| 収録物識別子 | 20452322 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 関連識別子 | https://doi.org/10.1038/s41598-018-28690-6 | |||||
| 権利 | ||||||
| 権利情報 | © The Author(s) 2018 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 値 | 6833728 bytes | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 491.42 | |||||
| 出版者 | ||||||
| 出版者 | Nature Publishing Group | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 雑誌掲載論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Journal Article | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 01 | |||||
| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | https://www.nature.com/articles/s41598-018-28690-6 | |||||
