{"created":"2023-06-19T09:20:48.966262+00:00","id":34530,"links":{},"metadata":{"_buckets":{"deposit":"7c4747ee-4bd8-4e27-95bd-6b8edc525c35"},"_deposit":{"created_by":17,"id":"34530","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"34530"},"status":"published"},"_oai":{"id":"oai:kumadai.repo.nii.ac.jp:00034530","sets":["413:443"]},"author_link":["169456","169458","169457"],"item_16_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-18","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"50.e7","bibliographicPageStart":"40","bibliographicVolumeNumber":"103","bibliographic_titles":[{},{"bibliographic_title":"Experimental hematology","bibliographic_titleLang":"en"}]}]},"item_16_creator_3":{"attribute_name":"別言語の著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tsuruda, Mariko"}],"nameIdentifiers":[{"nameIdentifier":"169456","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Morino-Koga, Saori","creatorNameLang":"en"},{"creatorName":"古賀, 沙緒里","creatorNameLang":"ja"},{"creatorName":"コガ, サオリ","creatorNameLang":"ja-Kana"},{"creatorName":"森野 古賀, 沙緒里","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Morino-Koga","familyNameLang":"en"},{"familyName":"古賀","familyNameLang":"ja"},{"familyName":"コガ","familyNameLang":"ja-Kana"},{"familyName":"森野 古賀","familyNameLang":"ja"}],"givenNames":[{"givenName":"Saori","givenNameLang":"en"},{"givenName":"沙緒里","givenNameLang":"ja"},{"givenName":"サオリ","givenNameLang":"ja-Kana"},{"givenName":"沙緒里","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"169457","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Minetaro"}],"nameIdentifiers":[{"nameIdentifier":"169458","nameIdentifierScheme":"WEKO"}]}]},"item_16_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Definitive hematopoietic cells develop from the fetal liver kinase 1 (Flk1)+ mesodermal cells during the in vitro differentiation of mouse embryonic stem cells (ESCs). VE-cadherin+CD41-CD45- (V+41-45-) hemogenic endothelial cells (HECs) and VE-cadherin+CD41+CD45- (V+41+45-) cells mediate the definitive hematopoietic development from Flk1+ cells. Bone morphogenetic protein 4 (BMP4) is known to be essential for the formation of mesoderm. However, the role of BMP4 in the differentiation of the VE-cadherin+ definitive hematopoietic precursors from the mesoderm has been elusive. We addressed this issue using a co-aggregation culture of ESC-derived Flk1+ cells with OP9 stromal cells. This culture method induced V+41-45- cells, V+41+45- cells, and CD45+ cells from Flk1+ cells. V+41+45- cells possessed the potentials of erythromyeloid and T-lymphoid differentiation. When Flk1+ cells were cultured in the presence of a high concentration of BMP4, the generation of V+41-45- cells was enhanced. The increase of V+41-45- cells led to the subsequent increase of V+41+45- cells and CD45+ cells. The addition of BMP4 also increased hematopoietic colony-forming cells of various lineages. Furthermore, BMP4 promoted the expansion of V+41+45- cells independently of the preceding V+41-45- cell stage. These results suggest that BMP4 has promotive effects on the differentiation of V+41-45- HECs from Flk1+ mesodermal cells and the subsequent proliferation of V+41+45- hematopoietic precursors. These findings may provide insights for establishing a culture system to induce definitive hematopoietic stem cells from ESCs.","subitem_description_type":"Other"}]},"item_16_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"International Society for Experimental Hematology"}]},"item_16_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.exphem.2021.08.008","subitem_relation_type_select":"DOI"}}]},"item_16_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C)2021 ISEH - Society for Hematology and Stem Cells.This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/."},{"subitem_rights":"This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.exphem.2021.08.008."}]},"item_16_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-472X","subitem_source_identifier_type":"ISSN"}]},"item_16_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鶴田, 真理子"},{"creatorName":"ツルダ, マリコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"169456","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Morino-Koga, Saori","creatorNameLang":"en"},{"creatorName":"古賀, 沙緒里","creatorNameLang":"ja"},{"creatorName":"コガ, サオリ","creatorNameLang":"ja-Kana"},{"creatorName":"森野 古賀, 沙緒里","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Morino-Koga","familyNameLang":"en"},{"familyName":"古賀","familyNameLang":"ja"},{"familyName":"コガ","familyNameLang":"ja-Kana"},{"familyName":"森野 古賀","familyNameLang":"ja"}],"givenNames":[{"givenName":"Saori","givenNameLang":"en"},{"givenName":"沙緒里","givenNameLang":"ja"},{"givenName":"サオリ","givenNameLang":"ja-Kana"},{"givenName":"沙緒里","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"169457","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小川, 峰太郎"},{"creatorName":"オガワ, ミネタロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"169458","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-18"}],"displaytype":"detail","filename":"10-1016_j-exphem-202108008.pdf","filesize":[{"value":"5.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"10-1016_j-exphem-202108008","url":"https://kumadai.repo.nii.ac.jp/record/34530/files/10-1016_j-exphem-202108008.pdf"},"version_id":"d5a65865-8bdf-4319-a37c-bacb6d8093ee"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bone Morphogenetic Protein 4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"VE-cadherin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mouse Embryonic Stem Cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mesoderm","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hemogenic Endothelial Cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Stromal Cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bone morphogenetic protein 4 differently promotes distinct VE-cadherin+ precursor stages during the definitive hematopoietic development from embryonic stem cell-derived mesodermal cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bone morphogenetic protein 4 differently promotes distinct VE-cadherin+ precursor stages during the definitive hematopoietic development from embryonic stem cell-derived mesodermal cells","subitem_title_language":"en"}]},"item_type_id":"16","owner":"17","path":["443"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-19"},"publish_date":"2022-11-19","publish_status":"0","recid":"34530","relation_version_is_last":true,"title":["Bone morphogenetic protein 4 differently promotes distinct VE-cadherin+ precursor stages during the definitive hematopoietic development from embryonic stem cell-derived mesodermal cells"],"weko_creator_id":"17","weko_shared_id":17},"updated":"2023-08-18T07:21:32.951690+00:00"}