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Evaluation of polyamidoamine dendrimer/α-cyclodextrin conjugate (generation 3, G3) as a novel carrier for small interfering RNA (siRNA)
http://hdl.handle.net/2298/9109
http://hdl.handle.net/2298/91094da26f41-593f-46cb-ad80-73aa1a86f881
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Arima-JCR-R2-submit.pdf (221.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-08-08 | |||||
| タイトル | ||||||
| タイトル | Evaluation of polyamidoamine dendrimer/α-cyclodextrin conjugate (generation 3, G3) as a novel carrier for small interfering RNA (siRNA) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | small interfering RNA, cyclodextrin, dendrimer, delivery, luciferase | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
堤, 利仁
× 堤, 利仁× Hirayama, Fumitoshi× Uekama, Kaneto× Arima, Hidetoshi |
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| 別言語の著者 |
有馬, 英俊
× 有馬, 英俊 |
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| 内容記述 | ||||||
| 内容記述 | As the first step toward an evaluation of the potential use of the PAMAM dendrimer (G3) conjugate with α-cyclodextrin (α-CDE) for a small interfering RNA (siRNA) carrier, the ternary complexes of α-CDE or the transfection reagents such as LipofactamineTM2000 (L2), TransFastTM (TF) and LipofectinTM (LF) with plasmid DNA (pDNA) and siRNA were prepared, and their RNAi effects, cytotoxicity, physicochemical properties and intracellular distribution were compared. Here the pGL2- control vector (pGL2) and pGL3-control vector (pGL3) encoding the firefly luciferase gene and the two corresponding siRNAs (siGL2 and siGL3) were used. The ternary complexes of pGL3/siGL3/α-CDE showed the potent RNAi effects with negligible cytotoxicity compared to those of the transfection reagents in various cells. α-CDE strongly interacted with both pDNA and siRNA, and suppressed siRNA degradation by serum, compared to those of the transfection reagents. α-CDE allowed fluorescent labeled siRNA to distribute in cytoplasm, whereas the transfection reagents resided in both nucleus and cytoplasm in NIH3T3 cells. Furthermore, the binary complex of siRNA/α-CDE provided the significant RNAi effect in NIH3T3 cells transiently and stably expressing luciferase gene. These results suggest that α-CDE may be utilized as a novel carrier for siRNA. | |||||
| 書誌情報 |
Journal of Controlled Release 巻 119, 号 3, p. 349-359, 発行年 2007-06-22 |
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| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10458678 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1016/j.jconrel.2007.03.013 | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 01683659 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 221567 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 499 | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 01 | ||||||
| 著作の一部 | ||||||
| 関連名称 | http://www.elsevier.com/wps/find/journaldescription.cws_home/502690/description#description | |||||
