| Item type |
会議発表論文 / Conference Paper(1) |
| 公開日 |
2007-07-30 |
| タイトル |
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タイトル |
Biological Effects of Narrow Band Pulsed Electric Fields |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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|
主題 |
Narrow band pulsed electric field (NPEF), pulse modulated sinusoidal wave, CHO cell, intracellular effect, electroporation, DNA degradation, Acridine Orange assay |
| 資源タイプ |
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資源タイプ |
conference paper |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
勝木, 淳
野村, 尚之
古賀, 豪人
秋山, 秀典
内田, 一郎
安部, 眞一
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| 内容記述 |
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内容記述 |
This paper describes the process of narrow band pulsed electric fields (NPEFs) and its effect on mammalian cells. The NPEF consists of a pulse modulated sinusoidal wave (PMSW), which allows delivery of well-defined electric fields in terms of frequency, field strength and deposition energy to the biological systems. 100 μs long sinusoidal electric fields with a frequency of 0.02, 2 or 50 MHz and field strengths of up to 2 kV/cm are applied to CHO cells with variation in the DNA density in the cells investigated by means of Acridine Orange assay. The experiments indicate that 50 MHz fields cause DNA degradation without cell membrane defects, while 0.02 MHz fields lead to an increase in membrane permeability which is similar to the effect known as electroporation. The intermediate frequency of 2 MHz influences both the membrane and DNA. It is demonstrated that the MHz range narrowband electric fields with the amplitude level of 1 kV/cm cause intracellular effects in mammalian cells. |
| bibliographic_information |
en : IEEE Transactions on Dielectrics and Electrical Insulation
巻 14,
号 3,
p. 663-668,
発行日 2007-06
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| item_17_source_id_7 |
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|
収録物識別子 |
1070-9878 |
| item_17_relation_9 |
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識別子タイプ |
NCID |
|
|
関連識別子 |
BA26187046 |
| 権利 |
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言語 |
en |
|
権利情報 |
(C) 2007 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE. IEEE, IEEE Transactions on Dielectrics and Electrical Insulation, 14, 3, 2007, 663-668 |
| 出版タイプ |
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|
出版タイプ |
VoR |
| 日本十進分類法 |
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主題Scheme |
NDC |
|
主題 |
500 |
| 出版者 |
|
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出版者 |
Institute of Electrical and Electronics Engineers |
|
言語 |
en |
| 関連 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1109/TDEI.2007.369529 |
04225345.pdf (1.4 MB)
