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Plasminogen potentiates thrombin cytotoxicity and contributes to pathology of intracerebral hemorrhage in rats
http://hdl.handle.net/2298/10028
http://hdl.handle.net/2298/10028ae31bc1d-e6b4-4c4e-8e66-3695629f468c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
J Cereb B0028-0506.pdf (526.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-12-03 | |||||
| タイトル | ||||||
| タイトル | Plasminogen potentiates thrombin cytotoxicity and contributes to pathology of intracerebral hemorrhage in rats | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | collagenase, cortical cells, hemorrhagic brain injury, plasminogen, slice cultures, thrombin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
藤本, 真二
× 藤本, 真二× 香月, 博志× Onishi, Masatoshi× Takagi, Mikako× 久米, 利明× 赤池, 昭紀 |
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| 別言語の著者 |
藤本, 真二
× 藤本, 真二× 香月, 博志× 大西, 正俊× 高木, 美佳子× 久米, 利明× 赤池, 昭紀 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Thrombin and plasmin are serine proteases involved in blood coagulation and fibrinolysis, whose precursors are circulating in blood stream. These blood-derived proteases might play important roles in the pathogenesis of intracerebral hemorrhage by acting on brain parenchymal cells. We previously reported that thrombin induced delayed neuronal injury through extracellular signal-regulated kinase (ERK)-dependent pathways. Here, we investigated potential cytotoxic actions of plasminogen, a precursor protein of plasmin, using slice cultures prepared from neonatal rat brain and intracortical microinjection model in adult rats. Although plasminogen alone did not evoke prominent neuronal injury, plasminogen caused significant neuronal injury when combined with a moderate concentration of thrombin (30 U/mL) in the cerebral cortex of slice cultures. The cortical injury was prevented by tranexamic acid and aprotinin. The combined neurotoxicity of thrombin and plasminogen was also prevented by PD98059, an inhibitor of ERK pathway, as well as by other agents that have been shown to prevent cortical injury induced by a higher concentration (100 U/mL) of thrombin alone. Extracellular signal-regulated kinase phosphorylation after plasminogen exposure was localized in cortical astrocytes. Moreover, microinjection of plasminogen in vivo potentiated thrombin-induced cortical injury, and inhibition of plasmin ameliorated hemorrhage-induced neuronal loss in the cerebral cortex. These results suggest that plasminogen/plasmin system augmenting thrombin neurotoxicity participates in hemorrhagic cortical injury. | |||||
| 書誌情報 |
Journal of Cerebral Blood Flow and Metabolism 巻 28, 号 3, p. 506-515, 発行年 2008-03 |
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| ISSN | ||||||
| 収録物識別子 | 0271678X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10458430 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1038/sj.jcbfm.9600547 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 値 | 526863 bytes | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 491.17 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 雑誌掲載論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Journal Article | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 01 | |||||
| 部分である | ||||||
| 関連タイプ | isPartOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://hdl.handle.net/2298/9868 | |||||
| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.nature.com/jcbfm/index.html | |||||
| コメント | ||||||
| 値 | 平成18～19年度科学研究費補助金(基盤研究(C))研究成果報告書 課題番号:18590052『ミクログリアの活性化を介する黒質線条体系ニューロン変性の機序とその防御』pp.143-152に掲載 | |||||
