WEKO3
アイテム
Depolarizing stimuli cause persistent and selective loss of orexin in rat hypothalamic slice culture
http://hdl.handle.net/2298/15389
http://hdl.handle.net/2298/15389b9782dae-a4ec-4e8e-a1af-74b7d97e05dc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kurosu-rev_r.pdf (653.3 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-07-14 | |||||
| タイトル | ||||||
| タイトル | Depolarizing stimuli cause persistent and selective loss of orexin in rat hypothalamic slice culture | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | c-Fos, Depolarization, Dynorphin, Hypocretin, Hypothalamus, Melanin-concentrating hormone | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
香月, 博志
× 香月, 博志× Kurosu, Shinsuke× Michinaga, Shotaro× Hisatsune, Akinori× Isohama, Yoichiro× Izumi, Yasuhiko× 久米, 利明× 赤池, 昭紀 |
|||||
| 別言語の著者 |
香月, 博志
× 香月, 博志× 黒須, 真介× 道永, 昌太郎× 久恒, 昭哲× 礒濱, 洋一郎× 泉, 安彦× 久米, 利明× 赤池, 昭紀 |
|||||
| 内容記述 | ||||||
| 内容記述 | A hypothalamic neuropeptide orexin (hypocretin) is a critical regulator of physiological processes including sleep/wakefulness and feeding. Using organotypic slice culture of rat hypothalamus, we found that exposure to elevated extracellular concentration of K+ (+10-30 mM) for 24-72 h led to a substantial decrease in the number of neurons immunoreactive for orexin and a co-existing neuropeptide dynorphin-A. In contrast, the same treatment affected neither the number of melanin-concentrating hormone-immunoreactive neurons nor the number of total neurons. A substantial decrease of orexin-immunoreactive neurons was also induced by 72 h treatment with 1-10 μM veratridine, a Na+ channel activator. The effect of elevated K+ was only partially reversible, and that of veratridine was virtually irreversible, although the decrease in orexin immunoreactivity was not associated with signs of cell damage assessed by propidium iodide uptake and Hoechst 33342 nuclear staining. In addition, the level of preproorexin mRNA did not decrease during treatment with elevated K+ or veratridine. After treatment with elevated K+ and veratridine, c-Fos immunoreactivity appeared in orexin-immunoreactive neurons but not in melanin-concentrating hormone-immunoreactive neurons, suggesting selective excitation of orexin neurons. However, the amount of orexin released extracellularly was paradoxically decreased by treatment with elevated K+ and veratridine. Overall, these characteristics of orexin neurons may be taken into consideration to understand the behaviors of these neurons under physiological and pathophysiological conditions. | |||||
| 書誌情報 |
Peptides 巻 31, 号 6, p. 1131-1138, 発行年 2010-06 |
|||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1016/j.peptides.2010.03.006 | |||||
| 権利 | ||||||
| 権利情報 | © 2010 Elsevier Inc. | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 01969781 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 653265 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 491.17 | |||||
| 出版者 | ||||||
| 出版者 | Elsevier Inc. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 01 | ||||||
| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T0M-4YN5P64-1&_user=4545557&_coverDate=06%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000008298&_version=1&_urlVersion=0&_userid=4545557&md5=88271472653e993e41bb078e216cb9ec | |||||
