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Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signaling
http://hdl.handle.net/2298/25781
http://hdl.handle.net/2298/2578155225a12-4793-4c2f-a949-510674c9c351
| 名前 / ファイル | ライセンス | アクション |
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Biochemical J. ,441,2,719-730, supp.pdf (1.1 MB)
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Biochemical J. ,441,2,719-730.pdf (2.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-10-30 | |||||
| タイトル | ||||||
| タイトル | Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signaling | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Nitric oxide, oxidative stress, reactive oxygen species, mitochondria, ROS signaling, peroxynitrite | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Ahmed, Khandaker Ahtesham
× Ahmed, Khandaker Ahtesham× 澤, 智裕× Ihara, Hideshi× Kasamatsu, Shingo× Yoshitake, Jun× Rahaman, Md. Mizanur× 岡本, 竜哉× 藤井, 重元× 赤池, 孝章 |
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| 別言語の著者 |
澤, 智裕
× 澤, 智裕× 居原, 秀× 笠松, 真吾× 吉武, 淳× 岡本, 竜哉× 藤井, 重元× 赤池, 孝章 |
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| 内容記述 | ||||||
| 内容記述 | 8-Nitro-cGMP (8-nitroguanosine 3′,5′-cyclic monophosphate) is a nitrated derivative of cGMP, which can function as a unique electrophilic second messenger involved in regulation of an antioxidant adaptive response in cells. In the present study, we investigated chemical and biochemical regulatory mechanisms involved in 8-nitro-cGMP formation, with particular focus on the roles of ROS (reactive oxygen species). Chemical analyses demonstrated that peroxynitrite-dependent oxidation and myeloperoxidase-dependent oxidation of nitrite in the presence of H 2O 2 were two major pathways for guanine nucleotide nitration. Among the guanine nucleotides examined, GTP was the most sensitive to peroxynitrite-mediated nitration. Immunocytochemical and tandem mass spectrometric analyses revealed that formation of 8-nitro-cGMP in rat C6 glioma cells stimulated with lipopolysaccharide plus pro-inflammatory cytokines depended on production of both superoxide and H 2O 2. Using the mitochondria-targeted chemical probe MitoSOX™ Red, we found that mitochondria-derived superoxide can act as a direct determinant of 8-nitro-cGMP formation. Furthermore, we demonstrated that Nox2 (NADPH oxidase 2)-generated H 2O 2regulated mitochondria-derived superoxide production, which suggests the importance of cross-talk between Nox2-dependent H 2O 2 production and mitochondrial superoxide production. The results of the present study suggest that 8-nitro-cGMP can serve as a unique second messenger thatmay be implicated in regulating ROS signalling in the presence of NO. | |||||
| 書誌情報 |
Biochemical Journal 巻 441, 号 2, p. 719-730, 発行年 2012-01-15 |
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| ISSN | ||||||
| 収録物識別子 | 02646021 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 21967515 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1042/BJ20111130 | |||||
| 権利 | ||||||
| 権利情報 | © 2012 Portland Press Ltd. | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 2899297 bytes | ||||||
| 形態 | ||||||
| 1076196 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 491 | |||||
| 出版者 | ||||||
| 出版者 | Portland Press Ltd. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
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| text | ||||||
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| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.biochemj.org/bj/441/bj4410719.htm | |||||
