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Polypseudorotaxanes of Pegylated α-Cyclodextrin/Polyamidoamine Dendrimer Conjugate with Cyclodextrins as a Sustained Release System for DNA
http://hdl.handle.net/2298/26022
http://hdl.handle.net/2298/260224a258a55-6540-4a22-a0e2-9d7a68c734dc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Bioorganic and Medicinal Chemistry2012.VOL20-4.pdf (640.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-12-11 | |||||
| タイトル | ||||||
| タイトル | Polypseudorotaxanes of Pegylated α-Cyclodextrin/Polyamidoamine Dendrimer Conjugate with Cyclodextrins as a Sustained Release System for DNA | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | cyclodextrin, polypseudorotaxane, dendrimer, DNA, sustained release | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Motoyama, Keiichi
× Motoyama, Keiichi× Hayashida, Kayoko× Higashi, Taishi× Arima, Hidetoshi |
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| 別言語の著者 |
本山, 敬一
× 本山, 敬一× 林田, 佳代子× 東, 大志× 有馬, 英俊 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Nonviral gene delivery suffers from a number of limitations including short transgene expression times and low transfection efficiency. In this study, we examined whether polypseudorotaxanes (PPRXs) of polyethylene glycol (PEG, molecular weight: 2,000)-grafted α-cyclodextrin (α-CyD)/ polyamidoamine dendrimer conjugate (PEG-α-CDE) with CyDs have the potential for the novel sustained release systems for plasmid DNA (pDNA). The PEG-α-CDE/pDNA complex formed PPRXs with α-CyD and γ-CyD solutions, but not with β-CyD solution. In the PEG-α-CDE/CyDs PPRX systems, 20.6 mol of α-CyD and 11.8 mol of γ-CyD were involved in the PPRXs formation with one PEG chain by α-CyD and γ-CyD, respectively, consistent with in the PEG-dendrimer/CyDs systems. PEG-α-CDE/pDNA/α-CyD PPRX and PEG-α-CDE/pDNA/γ-CyD PPRX formed hexagonal and tetragonal columnar channels in the crystalline phase, respectively. In addition, the CyDs PPRX provided the sustained release of pDNA from PEG-α-CDE complex with pDNA at least 72 h in vitro. The release of pDNA from CyDs PPRX retarded as the volume of dissolution medium decreased. Furthermore, the PEG-α-CDE/γ-CyD PPRX system showed sustained transfection efficiency after intramuscular injection to mice at least for 14 days. These results suggest that the PEG-α-CDE/CyD PPRX systems are useful for novel sustained DNA release systems. | |||||
| 書誌情報 |
Bioorganic and Medicinal Chemistry 巻 20, 号 4, p. 1425-1433, 発行年 2012-02-15 |
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| ISSN | ||||||
| 収録物識別子 | 09680896 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 22277591 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1016/j.bmc.2011.12.060 | |||||
| 権利 | ||||||
| 権利情報 | © 2012 Elsevier Limited | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 値 | 640544 bytes | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 491.7 | |||||
| 出版者 | ||||||
| 出版者 | Elsevier Limited | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 雑誌掲載論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Journal Article | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 01 | |||||
| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.sciencedirect.com/science/article/pii/S0968089612000028 | |||||
