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M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立
http://hdl.handle.net/2298/27461
http://hdl.handle.net/2298/2746184c0f86e-da97-4402-b63b-3a0a448941bf
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
MH0009_027-038.pdf (1.6 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-04-18 | |||||
| タイトル | ||||||
| タイトル | M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題 | Tet-Off system, M12 cell, TRAF3, NF-kB | |||||
| 資源タイプ | ||||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
田邊, 香野
× 田邊, 香野× 坂本, 瞳× 乾, 誠治 |
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| 別言語の著者 |
田邊, 香野
× 田邊, 香野× 坂本, 瞳× 乾, 誠治 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | IL-4 induces IgE class switching in B cells together with CD40 stimulation. Studies on the signal transduction mechanism downstream of IL-4 receptor have revealed that NF-κB as well as Jak-STAT pathway plays an important role. We showed the possibility that alternative pathway of NF-κB was activated after IL-4 stimulation of murine B cell line M12. Alternative pathway of NF-κB is strictly regulated by constant degradation of NIK, an essential molecule for NF-κB activation. TRAF3 is the key molecule to keep the alternative pathway in an inactive state. TRAF3 is also regulated by the constant degradation by ubiquitin-proteasome system. To elucidate the mechanism of the TRAF3 degradation, we prepared Tet-Off gene expression system in M12 to inducibly express TRAF3 which was toxic when overexpressed in cells. Firstly, pTet-Off vector was introduced into M12 cells by electroporation and G418 resistant clones were selected which harbored pTet-Off. Clones were further selected to show high responsiveness to Doxycyclin withdrawal using transient expression of luciferase vector. One of the clones, C'' , was transfected with pTRE2pur-mTRAF3 vector to obtain FLAG-tagged mTRAF3 expressing cells upon induction. One clone, C'' -1' , was successfully established which expressed mTRAF3 in an inducible manner. | |||||
| 書誌情報 |
熊本大学医学部保健学科紀要 巻 9, p. 27-38, 発行年 2013-03-30 |
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| ISSN | ||||||
| 収録物識別子 | 18807151 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12010572 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 1588446 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 463 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | Establishment of an inducible M12-Tet-Off pTRE2pur FLAG mTRAF3 cell | |||||
| タイトル（ヨミ） | ||||||
| その他のタイトル | M12 Tet Off pTRE2pur FLAG mTRAF3 サイボウカブ ノ ジュリツ | |||||
| 出版者 | ||||||
| 出版者 | 熊本大学 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 紀要論文 | ||||||
| 資源タイプ・NII | ||||||
| Departmental Bulletin Paper | ||||||
| 資源タイプ・DCMI | ||||||
| text | ||||||
| 資源タイプ・ローカル表示コード | ||||||
| 02 | ||||||
