| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2013-04-18 |
| タイトル |
|
|
タイトル |
M12-Tet-Off pTRE2pur FLAG mTRAF3細胞株の樹立 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
M12 Tet Off pTRE2pur FLAG mTRAF3 サイボウカブ ノ ジュリツ |
|
言語 |
ja-Kana |
| タイトル |
|
|
タイトル |
Establishment of an inducible M12-Tet-Off pTRE2pur FLAG mTRAF3 cell |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
Tet-Off system, M12 cell, TRAF3, NF-kB |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 |
田邊, 香野
坂本, 瞳
乾, 誠治
|
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
IL-4 induces IgE class switching in B cells together with CD40 stimulation. Studies on the signal transduction mechanism downstream of IL-4 receptor have revealed that NF-κB as well as Jak-STAT pathway plays an important role. We showed the possibility that alternative pathway of NF-κB was activated after IL-4 stimulation of murine B cell line M12. Alternative pathway of NF-κB is strictly regulated by constant degradation of NIK, an essential molecule for NF-κB activation. TRAF3 is the key molecule to keep the alternative pathway in an inactive state. TRAF3 is also regulated by the constant degradation by ubiquitin-proteasome system. To elucidate the mechanism of the TRAF3 degradation, we prepared Tet-Off gene expression system in M12 to inducibly express TRAF3 which was toxic when overexpressed in cells. Firstly, pTet-Off vector was introduced into M12 cells by electroporation and G418 resistant clones were selected which harbored pTet-Off. Clones were further selected to show high responsiveness to Doxycyclin withdrawal using transient expression of luciferase vector. One of the clones, C'' , was transfected with pTRE2pur-mTRAF3 vector to obtain FLAG-tagged mTRAF3 expressing cells upon induction. One clone, C'' -1' , was successfully established which expressed mTRAF3 in an inducible manner. |
| 書誌情報 |
ja : 熊本大学医学部保健学科紀要
巻 9,
p. 27-38,
発行年 2013-03-30
|
| ISSN |
|
|
収録物識別子 |
1880-7151 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12010572 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
463 |
| 出版者 |
|
|
出版者 |
熊本大学 |
|
言語 |
ja |
MH0009_027-038.pdf (1.6 MB)
