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Bone morphogenetic protein 4 differently promotes distinct VE-cadherin+ precursor stages during the definitive hematopoietic development from embryonic stem cell-derived mesodermal cells
http://hdl.handle.net/2298/00045963
http://hdl.handle.net/2298/00045963f75df183-c047-460b-8e2a-1d88015b0cf2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
10-1016_j-exphem-202108008 (5.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-11-19 | |||||
| タイトル | ||||||
| タイトル | Bone morphogenetic protein 4 differently promotes distinct VE-cadherin+ precursor stages during the definitive hematopoietic development from embryonic stem cell-derived mesodermal cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Bone Morphogenetic Protein 4, VE-cadherin, Mouse Embryonic Stem Cells, Mesoderm, Hemogenic Endothelial Cells, Stromal Cells | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
鶴田, 真理子
× 鶴田, 真理子× 古賀, 沙緒里× 小川, 峰太郎 |
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| 別言語の著者 |
Tsuruda, Mariko
× Tsuruda, Mariko× 古賀, 沙緒里× Ogawa, Minetaro |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Definitive hematopoietic cells develop from the fetal liver kinase 1 (Flk1)+ mesodermal cells during the in vitro differentiation of mouse embryonic stem cells (ESCs). VE-cadherin+CD41-CD45- (V+41-45-) hemogenic endothelial cells (HECs) and VE-cadherin+CD41+CD45- (V+41+45-) cells mediate the definitive hematopoietic development from Flk1+ cells. Bone morphogenetic protein 4 (BMP4) is known to be essential for the formation of mesoderm. However, the role of BMP4 in the differentiation of the VE-cadherin+ definitive hematopoietic precursors from the mesoderm has been elusive. We addressed this issue using a co-aggregation culture of ESC-derived Flk1+ cells with OP9 stromal cells. This culture method induced V+41-45- cells, V+41+45- cells, and CD45+ cells from Flk1+ cells. V+41+45- cells possessed the potentials of erythromyeloid and T-lymphoid differentiation. When Flk1+ cells were cultured in the presence of a high concentration of BMP4, the generation of V+41-45- cells was enhanced. The increase of V+41-45- cells led to the subsequent increase of V+41+45- cells and CD45+ cells. The addition of BMP4 also increased hematopoietic colony-forming cells of various lineages. Furthermore, BMP4 promoted the expansion of V+41+45- cells independently of the preceding V+41-45- cell stage. These results suggest that BMP4 has promotive effects on the differentiation of V+41-45- HECs from Flk1+ mesodermal cells and the subsequent proliferation of V+41+45- hematopoietic precursors. These findings may provide insights for establishing a culture system to induce definitive hematopoietic stem cells from ESCs. | |||||
| 書誌情報 |
en : Experimental hematology 巻 103, p. 40-50.e7, 発行年 2021-11-18 |
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| ISSN | ||||||
| 収録物識別子 | 0301-472X | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1016/j.exphem.2021.08.008 | |||||
| 権利 | ||||||
| 権利情報 | (C)2021 ISEH - Society for Hematology and Stem Cells.This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. | |||||
| 権利 | ||||||
| 権利情報 | This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.exphem.2021.08.008. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 出版者 | ||||||
| 出版者 | International Society for Experimental Hematology | |||||
